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D9564

Sigma-Aldrich

DAPI, dilactate

≥98% purity (HPLC), powder

Synonym(s):

4′,6-Diamidino-2-phenyindole, dilactate

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About This Item

Empirical Formula (Hill Notation):
C16H15N5 · 2C3H6O3
Molecular Weight:
457.48
UNSPSC Code:
12171500
PubChem Substance ID:
329798905
NACRES:
NA.47

product name

DAPI, dilactate, ≥98% (HPLC)

Quality Level

300

Assay

≥98% (HPLC)

form

powder

color

yellow

solubility

H2O: 20 mg/mL

ε (extinction coefficient)

28.00-32.50 at 345-351 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)

InChI key

LCAUPANIXBMNIA-UHFFFAOYSA-N

Related Categories

General description

DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.

Application

DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.

Biochem/physiol Actions

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

related product

Product No.
Description
Pricing

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. Which filter sets can be used to visualize the fluorophore produced by Product D9564, DAPI, dilactate (DAPI-DNA)?

    One such filter set is the Zeiss Filter Set 01 (ex=359 nm, em=461 nm), information about which is posted here.

  5. Isn't the hydrochloride salt form of DAPI a more sensitive detector of DNA than Product D9564, DAPI, dilactate (DAPI-DNA)?

    The dilactate salt has as much sensitivity in detection as the dihydrochloride salt, which we also offer as Product No. D9542.

  6. Can Product D9564, DAPI dilactate (DAPI-DNA), be used as a vital stain?

    As we note in the Product Information Sheet for the Dihydrochloride salt of DAPI, Product No. D9542, DAPI can be used as a vital dye to stain mature pollen grains, but we have tested it as a vital stain in other cell types.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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