C7698
Cycloheximide
from microbial, ≥94% (TLC)
Synonym(s):
3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide, Actidione, Naramycin A
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About This Item
Empirical Formula (Hill Notation):
C15H23NO4
CAS Number:
Molecular Weight:
281.35
Beilstein:
88868
EC Number:
MDL number:
UNSPSC Code:
12352111
PubChem Substance ID:
NACRES:
NA.77
biological source
microbial
Quality Level
Assay
≥94% (TLC)
form
powder
color
white to off-white
solubility
ethanol: soluble, clear to hazy
antibiotic activity spectrum
fungi
yeast
Mode of action
protein synthesis | interferes
storage temp.
2-8°C
SMILES string
[H][C@]1(C[C@@H](C)C[C@H](C)C1=O)[C@H](O)CC2CC(=O)NC(=O)C2
InChI
1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
InChI key
YPHMISFOHDHNIV-FSZOTQKASA-N
Gene Information
human ... FKBP1A(2280) , PIN1(5300)
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General description
Cycloheximide, also known as Actidione, is a glutarimide antibiotic commonly derived from the bacterium Streptomyces griseus. It acts as a potent inhibitor of protein biosynthesis in eukaryotic cells by disrupting the translocation step during translation, effectively blocking translational elongation. In microbiology, it plays a crucial role as a selection agent for resistant strains of yeast and fungi, proving invaluable in controlled experimental environments.
In cell biology and biochemical research, Cycloheximide showcases a dual nature concerning Apoptosis Induction, inducing or inhibiting apoptosis depending on the cell type. Its rapid and reversible effects make it an ideal choice for studying cellular processes and determining protein half-life. Cycloheximide also finds extensive applications in biomedical research, where it inhibits protein synthesis in eukaryotic cells studied in vitro (outside of organisms) and its effects are rapidly reversed by simply removing it. This makes Cycloheximide a go-to choice for exploring cell biology, biomedical and biochemical research, offering precise control and versatility in experiments.
In cell biology and biochemical research, Cycloheximide showcases a dual nature concerning Apoptosis Induction, inducing or inhibiting apoptosis depending on the cell type. Its rapid and reversible effects make it an ideal choice for studying cellular processes and determining protein half-life. Cycloheximide also finds extensive applications in biomedical research, where it inhibits protein synthesis in eukaryotic cells studied in vitro (outside of organisms) and its effects are rapidly reversed by simply removing it. This makes Cycloheximide a go-to choice for exploring cell biology, biomedical and biochemical research, offering precise control and versatility in experiments.
Application
- In yeast strains, cycloheximide has been used as a protein synthesis inhibitor in the cycloheximide chase experiment.
- It has been used to inhibit translation in mammalian cells.
- It has been used to suppress fungal growth.
Biochem/physiol Actions
Mode of Action: Translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for the selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.
Activity Spectrum: Active against yeast and fungi like Candida, Aspergillus, Saccharomyces, Penicillium
Activity Spectrum: Active against yeast and fungi like Candida, Aspergillus, Saccharomyces, Penicillium
Features and Benefits
- High-quality antibiotic suitable for multiple research applications
- Commonly used in Cell Biology and Biochemical applications
Storage and Stability
Tightly closed. Dry. Keep in a well-ventilated place. Keep locked up or in an area accessible
only to qualified or authorized persons.
only to qualified or authorized persons.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Oral - Aquatic Chronic 2 - Muta. 2 - Repr. 1B
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
监管及禁止进口产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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