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82950

Supelco

3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt

for spectrophotometric det. of Fe, 97.0%

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Synonym(s):
5,6-Diphenyl-3-(2-pyridyl)-1,2,4-triazine-4′,4″-disulfonic acid sodium salt, PDT disulfonate
Empirical Formula (Hill Notation):
C20H13N4NaO6S2
CAS Number:
69898-45-9
Molecular Weight:
492.46
EC Number:
274-196-3
MDL number:
MFCD00078417
UNSPSC Code:
12000000
PubChem Substance ID:
329769272
NACRES:
NA.21

Quality Level

100

Assay

97.0%

form

solid

quality

for spectrophotometric det. of Fe

technique(s)

UV/Vis spectroscopy: suitable

impurities

~7% water

mp

≥300 °C

cation traces

Co: ≤50 mg/kg
Cu: ≤50 mg/kg
Fe: ≤50 mg/kg
Mo: ≤50 mg/kg
Ni: ≤50 mg/kg
V: ≤50 mg/kg
Zn: ≤50 mg/kg

SMILES string

[Na+].OS(=O)(=O)c1ccc(cc1)-c2nnc(nc2-c3ccc(cc3)S([O-])(=O)=O)-c4ccccn4

InChI

1S/C20H14N4O6S2.Na/c25-31(26,27)15-8-4-13(5-9-15)18-19(14-6-10-16(11-7-14)32(28,29)30)23-24-20(22-18)17-3-1-2-12-21-17;/h1-12H,(H,25,26,27)(H,28,29,30);/q;+1/p-1

InChI key

ZGVNYCXXBQPDPQ-UHFFFAOYSA-M

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General description

3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt also known as Ferrozine is a chelating agent for ferrous iron.

Application

It was used in determining of chelating activity of Fe2+ using Boyer and McCleary method.

Other Notes

Highly specific water-soluble reagent for the accurate determination of submicrogram levels of serum iron (pH 3.5-4.5, lmax 562 nm, A 29′000)

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WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Direct and indirect assays of serum iron with a sensitive new color reagent.
D Plaut et al.
The American journal of medical technology, 38(3), 82-89 (1972-03-01)
F Ceriotti et al.
Clinical chemistry, 26(2), 327-331 (1980-02-01)
Serum iron is released from transferrin and reduced at pH 1.7 by treating serum with a 10 g/L ascorbic acid solution in 0.1 mol/L HCl. When ferrozine is added to this reagent, it forms a complex with iron that is
Molecular characterization of a putative Arabidopsis thaliana copper transporter and its yeast homologue.
Kampfenkel, Karlheinz, et al.
The Journal of Biological Chemistry, 270.47, 28479-28486 (1995)
K.L. Stookey
Analytical Chemistry, 42, 779-779 (1970)
Antioxidative activity of protein hydrolysate from round scad muscle using alcalase and flavourzyme.
Thiansilakul, Yaowapa, Soottawat Benjakul, and Fereidoon Shahidi.
Journal of Food Biochemistry, 31.2 , 266-287 (2007)
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