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Bathophenanthrolinedisulfonic acid disodium salt trihydrate

for the spectrophotometric det. of Fe, ≥98.0% (HPLC)

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Synonym(s):
4,7-Diphenyl-1,10-phenanthroline-disulfonic acid disodium salt trihydrate, Disodium bathophenanthrolinedisulfonate trihydrate
Empirical Formula (Hill Notation):
C24H14N2Na2O6S2 · 3H2O
CAS Number:
Molecular Weight:
590.53
EC Number:
UNSPSC Code:
23151817
NACRES:
NA.21

Quality Level

Assay

≥98.0% (HPLC)

form

solid

quality

for the spectrophotometric det. of Fe

technique(s)

UV/Vis spectroscopy: suitable

cation traces

Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
Mg: ≤10 mg/kg
Mn: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

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Application

Bathophenanthrolinedisulfonic acid disodium salt trihydrate was used in double electrochemical covalent coupling method for the fabrication of sensitive amperometric immunosensor, which was based on electro-click chemistry and diazonium chemistry. It was also used in an experimental study based upon, correlating the iron content with the activity, preparation and reconstituting the apoenzyme phenylamine hydroxylase.

Other Notes

Water soluble reagent for the determination of iron; in serum; For the determination of iron(II) by thermal lensing spectrophotometry

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Determination of iron with bathophenanthroline without deproteinisation.
J Bouda
Clinica chimica acta; international journal of clinical chemistry, 21(1), 159-160 (1968-07-01)
K. Miyaishi et al.
Analytica Chimica Acta, 124, 381-381 (1981)
D. Blair et al.
Talanta, 7, 163-163 (1961)
D W Gottschall et al.
The Journal of biological chemistry, 257(2), 845-849 (1982-01-25)
Phenylalanine hydroxylase requires 1.0 mol of iron/Mr = 50,000 subunit for maximal activity. A direct correlation between iron content and specific activity has been demonstrated through a comparison of enzyme activity and iron bound per subunit for various enzyme preparations
Double electrochemical covalent coupling method based on click chemistry and diazonium chemistry for the fabrication of sensitive amperometric immunosensor.
Honglan Q
Analytica Chimica Acta, 729, 28-34 (2013)

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