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77619

Supelco

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 125:24:1

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About This Item

CAS Number:
136112-00-0
MDL number:
MFCD00133763
UNSPSC Code:
12000000
PubChem Substance ID:
329767819
NACRES:
ND.02

grade

for molecular biology

Quality Level

100

product line

BioUltra

form

liquid

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

4-5(H2O-phase, after extraction with H2O, 1:1)

density

1.12 g/mL at 20 °C

λ

neat

UV absorption

λ: 330 nm Amax: 0.2
λ: 405 nm Amax: 0.1
λ: 510 nm Amax: 0.1

storage temp.

2-8°C

SMILES string

ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1

InChI

1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H

InChI key

ZYWFEOZQIUMEGL-UHFFFAOYSA-N

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Application


  • Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems: This research explores the application of the Phenol – chloroform – isoamyl alcohol mixture in extracting high-quality RNA for advanced therapeutic formulations, emphasizing its critical role in nanotechnology and pharmaceutical sciences (Lokras et al., 2022).

  • Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry: Demonstrates the use of the Phenol – chloroform – isoamyl alcohol mixture for precise extraction of microRNAs from biological fluids, contributing to cancer diagnostics and molecular pathology (Pero-Gascon et al., 2018).

  • The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs: Highlights the integration of the Phenol – chloroform – isoamyl alcohol mixture with novel technologies for forensic applications, enhancing DNA extraction processes critical in legal and criminal investigations (Nori and McCord, 2015).

  • Frequency of Epstein-Barr virus DNA sequences in human gliomas: Utilizes the Phenol – chloroform – isoamyl alcohol mixture for DNA extraction in medical research, facilitating the study of viral presence in cancer tissues and its implications for oncology and virology (Fonseca et al., 2015).

  • The critical role of DNA extraction for detection of mycobacteria in tissues: This study underscores the effectiveness of the Phenol – chloroform – isoamyl alcohol mixture in the molecular detection of pathogens, serving as a fundamental technique in microbiological diagnostics and infectious disease research (Radomski et al., 2013).

Physical form

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 125:24:1 (v/v/v); saturated with 2 M NaOAc pH 4

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

Target Organs

Liver,Kidney, Nervous system,Kidney,Liver,Skin

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品
易制毒化学品（2类）

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