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Merck
CN

70135

DCLS Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Deoxycholate Citrate Lactose Sucrose Agar

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171607

Key Documents

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Product Name

DCLS Agar, suitable for microbiology, NutriSelect® Plus

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12.0 g/L
lactose, 5.0 g/L
neutral red, 0.03 g/L
sodium citrate, 10.5 g/L
sodium desoxycholate, 2.5 g/L
sodium thiosulfate, 5.0 g/L
special peptone, 10.0 g/L
sucrose, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

selective and differential for Salmonella spp.
selective and differential for Shigella spp.
selective and differential for Vibrio spp.
selective and differential for Yersinia spp.

Quality Level

200

Application

A medium for the culture of pathogenic Vibrio species as well as Salmonella and Shigella.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 50 g in 1 litre of distilled water. Bring to the boil to dissolve the medium completely. Cool to 50°C and pour plates. Do not autoclave!

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCBW4222

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[Effectivity of Leifson agar modified by addition of sucrose. A comparison with the not-modified substrate (author's transl)].
F Burkhardt
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie, 239(4), 488-492 (1977-12-01)

Articles

Differentiation and ID Media for Salmonella

Salmonella, with 2,300+ serotypes, causes half of food-borne illnesses, often from dairy, poultry, and eggs.

Vibrio Detection

Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater.

沙门氏菌分离和鉴定培养基

沙门氏菌污染是全球食源性疾病的第二大成因。控制沙门氏菌的暴发是食品监管机构、餐馆和整个食品工业的一项重要任务。沙门氏菌家族包括2300多种血清型的细菌，但其中有两种类型，即肠炎沙门氏菌和鼠伤寒沙门氏杆菌，约占所有人类感染的一半。大多数沙门氏菌的暴发可以追溯到乳制品、家禽和肉制品，但沙门氏菌几乎可以在任何食物上生长。鸡、鸡蛋及其衍生产品的风险特别高。

弧菌检测

大多数弧菌采用葡萄糖等简单的 C 源作为能源，需要2-4％的NaCl或其他盐以及海水中存在的微量元素，即可实现最佳生长。有些物种可以使用多种能源。

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