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Millipore

MRS Broth

suitable for microbiology, NutriSelect® Basic

Synonym(s):

Lactobacillus Broth acc. to De Man, Rogosa and Sharpe

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.74

sterility

non-sterile

Quality Level

100

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dipotassium hydrogen phosphate, 2 g/L
glucose, 20 g/L
magnesium sulfate monohydrate, 0.2 g/L
manganous sulfate tetrahydrate, 0.05 g/L
meat extract, 8 g/L
peptone, 10 g/L
sodium acetate, 5 g/L
triammonium citrate, 2 g/L
yeast extract, 4 g/L

packaging

pkg of 500 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

pH

6.2±0.2

application(s)

agriculture
bioburden testing
environmental
food and beverages
microbiology

suitability

selective by low pH for Bifidobacterium spp.
selective by low pH for Lactobacillus spp.

Related Categories

General description

MRS Broth is used for the enrichment, cultivation, and isolation of all species of Lactobacillus from all types of material according to DeMan, Rogosa, and Sharpe. The medium can be used for confirmatory tests of colonies isolated from MRS Agar. The MRS culture media contain polysorbate (Tween 80), acetate, magnesium, and manganese which are known to act as special growth factors for Lactobacilli as well as a rich nutrient base. As these media show a very low degree of selectivity Pediococcus and Leuconostoc species, as well as other secondary bacteria, may grow on them. Most of the accompanying microflora can be inhibited by thallium acetate, sorbic acid, acetic acid, sodium nitrite, cycloheximide, and polymyxin. These substances can be used at varying concentrations and combinations, but inevitably a compromise must be reached between selectivity and recovery rate of the organism.

Application

MRS Broth is used for the enrichment, cultivation and isolation of all species of Lactobacillus from all types of material

Preparation Note

Dissolve 51.25 g in 1 litre distilled water and add 1 ml Tween® 80 (Cat. No. P8074). Boil to dissolve the medium completely. Fill into containers and sterilize by autoclaving at 121°C for 15 minutes. lncubate the culture up to 3 days at 35°C or up to 5 days at 30°C. If possible, incubate the culture in a CO2 enriched atmosphere in an anaerobic jar.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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L.C. Laleye et al.
Journal of Food Protection, 50, 1009-1009 (1987)
P Laloi et al.
Applied microbiology and biotechnology, 36(2), 196-204 (1991-11-01)
Whole cells of Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397 were able to hydrolyse alpha- and beta-caseins. Irrespective of the growth medium used, milk or De Man-Rogosa-Sharpe (MRS) broth, identical patterns of alpha- and beta-casein hydrolytic products, respectively, were visualized by
Rupal Mistry et al.
Genetics, 206(2), 889-904 (2017-04-18)
Resident gut bacteria are constantly influencing the immune system, yet the role of the immune system in shaping microbiota composition during an organism's life span has remained unclear. Experiments in mice have been inconclusive due to differences in husbandry schemes
J.D. DeMan et al.
The Journal of Applied Bacteriology, 23, 130-130 (1960)
Sonja Blasche et al.
Nature microbiology, 6(2), 196-208 (2021-01-06)
Microbial communities often undergo intricate compositional changes yet also maintain stable coexistence of diverse species. The mechanisms underlying long-term coexistence remain unclear as system-wide studies have been largely limited to engineered communities, ex situ adapted cultures or synthetic assemblies. Here

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