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Safety Information

67309

Millipore

Kovac′s reagent for indoles

suitable for microbiology

Synonym(s):

4-(Dimethylamino)benzaldehyde solution

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100 ML
CN¥650.07

CN¥650.07

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100 ML
CN¥650.07

About This Item

Empirical Formula (Hill Notation):
C9H11NO
CAS Number:
100-10-7
Molecular Weight:
149.19
Beilstein:
4132845
MDL number:
MFCD00003381
UNSPSC Code:
41171621
PubChem Substance ID:
329760541
NACRES:
NA.85

CN¥650.07

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Agency

according to ISO 16654:2001

Quality Level

200

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

4-(dimethlyamino)benzaldehyde, 50 g/L
hydrochloric acid, 240 g/L
isoamylic alcohol, 710 g/L

technique(s)

microbe id | specific enzyme detection: suitable

application(s)

agriculture
clinical testing
environmental
food and beverages
microbiology

storage temp.

2-8°C

suitability

Escherichia coli
coliforms

SMILES string

[H]C(=O)c1ccc(cc1)N(C)C

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This Item
33130D2004109762
Kovac’s reagent for indoles suitable for microbiology

67309

Kovac′s reagent for indoles

4-(Dimethylamino)benzaldehyde puriss. p.a., reag. Ph. Eur., ≥99% (perchloric acid titration)

33130

4-(Dimethylamino)benzaldehyde

4-(Dimethylamino)benzaldehyde suitable for histochemical demonstration of nitro blue tetrazolium reduction in neutrophils

D2004

4-(Dimethylamino)benzaldehyde

4-(Dimethylamino)benzaldehyde 98%

109762

4-(Dimethylamino)benzaldehyde

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

storage temp.

2-8°C

storage temp.

-

storage temp.

room temp

storage temp.

-

agency

according to ISO 16654:2001

agency

USP/NF, reag. Ph. Eur.

agency

-

agency

-

product line

BioChemika

product line

-

product line

-

product line

-

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

composition

4-(dimethlyamino)benzaldehyde, 50 g/L

composition

-

composition

-

composition

-

General description

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid.[1] For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.[2]

Application

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.
Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent.[3] It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.[4]

replaced by

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

109.4 °F

Flash Point(C)

43 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

易制毒化学品（3类）
危险化学品
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    Lot/Batch Number
    BCCL5138
    BCCK4337
    BCCH8824
    BCCG3914
    BCCF1994

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    Rita R. Colwell, R. Grigorova
    Methods in Microbiology, 19, 27-27 (1987)
    S. Harisha
    An Introduction to Practical Biotechnology, 193-193 (2005)
    R W Trepeta et al.
    Journal of clinical microbiology, 19(2), 172-174 (1984-02-01)
    Escherichia coli is the most common gram-negative microbe isolated and identified in clinical microbiology laboratories. It can be identified within 1 h by oxidase, indole, lactose, and beta-glucuronidase tests. The oxidase and indole tests are performed as spot tests, and
    Patrick R Porubsky et al.
    Archives of biochemistry and biophysics, 475(1), 14-17 (2008-04-22)
    Cytochrome P450 2A13 (CYP2A13) is a lung specific enzyme known to activate the potent tobacco procarcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) into two carcinogenic metabolites. CYP2A13 has been crystallized and X-ray diffraction experiments illuminated the structure of this enzyme, but with an unknown
    Rosario Zamora et al.
    Journal of agricultural and food chemistry, 52(13), 4166-4171 (2004-06-24)
    The Ehrlich reaction was optimized to determine the formation of pyrrolized phospholipids in edible oils in an attempt to understand the color reversion produced during the deodorization of poorly degummed edible oils. The procedure consisted of the treatment of the
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