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Merck
CN

08714

Methyl Red solution

suitable for microbiology

Synonym(s):

Methyl red indicator solution

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About This Item

Empirical Formula (Hill Notation):
C15H15N3O2
CAS Number:
Molecular Weight:
269.30
UNSPSC Code:
41171621
NACRES:
NA.85
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1843037
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Quality Level

agency

according to GB 4789.30-2016, according to ISO 22964:2017

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

dist. water, 200 mL , ethanol 95%, 300 mL , methyl red, 0.1 g

technique(s)

microbe id | metabolite detection: suitable

application(s)

clinical testing
environmental
food and beverages, microbiology

suitability

Enterococcus spp., Escherichia coli, Klebsiella spp., Proteus spp., Pseudomonas spp., Staphylococcus spp., bacteria

SMILES string

CN(C)c1ccc(cc1)\N=N\c2ccccc2C(O)=O

InChI

1S/C15H15N3O2/c1-18(2)12-9-7-11(8-10-12)16-17-14-6-4-3-5-13(14)15(19)20/h3-10H,1-2H3,(H,19,20)/b17-16+

InChI key

CEQFOVLGLXCDCX-WUKNDPDISA-N

General description

Methyl red solution is an azo dye which turns to red when pH is below 4.4 (yellow pH < 6.2, orange pH 4.4-6.2). Some bacteria utilize glucose to form large amounts of acid with the result that the pH value of the medium falls distinct. Other species produce no or less free acid. This difference can be visualized by using methyl red. This test is used to differentiate enteric bacteria.

Application

Methyl red solution is a pH indicator dye recommended for detection of bacteria producing acid from glucose fermentation such as Enterobacteriaceae in various samples.


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pictograms

FlameExclamation mark

signalword

Danger

hcodes

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

69.8 °F

flash_point_c

21 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

危险化学品

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Articles

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.

There are many other methods of detection to indicate the presence of E. coli. Review common tests and biochemical reactions for this contaminant.

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Related Content


X D Zhang et al.
Journal of hazardous materials, 170(2-3), 883-887 (2009-06-09)
An aqueous C.I. Acid Red 2 solution was decolorized by electrolysis using iron as anode. The decolorization mechanism was investigated through experimental observations on the electrochemical behavior of C.I. Acid Red 2 on Pt rotating disk electrode, UV-visible spectra of
Sofia Babanova et al.
Journal of bioscience and bioengineering, 112(4), 379-387 (2011-07-26)
Soluble artificial mediators are often applied to enhance the electron transfer from living cells to an anode in microbial fuel cells. Recently, we have demonstrated that the Candida melibiosica 2491 yeast strain possesses electrogenic properties and can be used as
Taiga Fujii et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 18(35), 10865-10872 (2012-07-26)
Asymmetric dye clusters with a single fluorophore (Cy3) and multiple quenchers (4'-methylthioazobenzene-4-carboxylate, methyl red, and 4'-dimethylamino-2-nitroazobenzene-4-carboxylate) were prepared. The dye and one-to-five quenchers were tethered through D-threoninol to opposite strands of a DNA duplex. NMR analysis revealed that the clusters



Global Trade Item Number

SKUGTIN
220868-10G04061836674878
08714-100ML-F04061838652805