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Merck
CN

53286

Brain Heart Infusion Broth

NutriSelect® Plus, according to ISO 6888, powder, for bacteria, pack of 100 g, pack of 500 g

Synonym(s):

BHI Broth, Brain Heart Broth, BHI Broth, Brain Heart Broth

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171607

Key Documents

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Product Name

Brain Heart Infusion Broth, NutriSelect® Plus, according to ISO 6888, powder, for bacteria, pack of 100 g, pack of 500 g

agency

according to ISO 6888

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

beef heart (infusion from 250g), 5 g/L
calf brains (infusion from 200g), 12.5 g/L
disodium hydrogen phosphate, 2.5 g/L
D(+)-glucose, 2 g/L
peptone, 10 g/L
sodium chloride, 5 g/L

packaging

pack of 100 g
pack of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

color

light yellow

final pH

7.4±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
life science and biopharma
microbiology
pathogen testing
pharmaceutical

suitability

Haemophilus spp.
bacteria
nonselective for Bacteroides spp.
nonselective for Candida spp.
nonselective for Escherichia coli
nonselective for Neisseria spp.
nonselective for Pseudomonas spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for Yersinia spp.
nonselective for bacteria (General Media)
nonselective for coliforms

Quality Level

200

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Application

Brain Heart Infusion Broth is recommended for the cultivation of fastidious pathogenic microorganisms and is often used for the examination of water, wastewater, meat, and food products. It is used as a culture medium forStreptococcus pneumonia, Listeria monocytogenes, Leishmania promastigotes and Clostridium difficile.

Disclaimer

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Features and Benefits

NutriSelect® Plus are powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

General description

Brain Heart Infusion Broth is recommended for the cultivation of various fastidious, pathogenic microorganisms, including yeasts and molds. The media is nutritious and well-buffered to support the growth of a wide range of microorganisms such as Streptococci, Pneumococci, Meningococci, etc. The media is especially suited for the cultivation of Staphylococci for the plasma coagulase test and setting up blood cultures. The addition of ascites permits the cultivation of Gonococci. The agar improves the growth of microaerophilic and anaerobic microorganisms. With the addition of 10% defibrinated sheep blood, it is suitable for the isolation and cultivation of Histoplasma capsulatum and other pathogenic fungi. Enzymatic digestion of animal tissues and brain-heart infusion provide amino acids, nitrogen, carbon, vitamins, and minerals for the growth of organisms. Glucose is the source of carbohydrates, and sodium chloride maintains the osmotic balance of the medium. Disodium phosphate is a buffering agent.

Other Notes

Production of 12α-hydroxysteroid dehydrogenase from Clostridium group P

Preparation Note

Dissolve 37 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCP0636
BCCM9831
BCCM7665
BCCL3710
BCCL9933

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Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism
Fachi J, et al.
Testing, 27(3), 750-761 (2019)
Comparison of culture media in the isolation and diagnosis of cutaneous leishmaniasis
Ahmet O, et al.
African Journal of Microbiology Research, 4(11), 1038-1043 (2010)
Kirsten Barnard et al.
Scientific reports, 9(1), 9117-9117 (2019-06-27)
The gut microbiota of mosquitoes is a crucial determinant of their fitness. As such, the biology of the gut microbiota of Anopheles arabiensis, a major malaria vector of Southern Africa, was investigated. Two laboratory strains of An. arabiensis were used;
Gene expression profiling of Listeria monocytogenes strain F2365 during growth in ultrahigh-temperature-processed skim milk
Liu Y and Ream A
Applied and Environmental Microbiology, 74(22), 6859-6866 (2008)
Economic evaluation of Streptococcus pneumoniae culture media
Morais V and Suarez N
American Journal of Biochemistry and Biotechnology, 12, 133-138 (2016)
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