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Merck
CN

41782

MRS Agar, Vegitone

suitable for microbiology, NutriSelect® Plus

Synonym(s):

DeMan, Rogosa and Sharpe Agar, Vegitone, Vegitone MRS Agar

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171605

Key Documents

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Product Name

MRS Agar, Vegitone, suitable for microbiology, NutriSelect® Plus

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
ammonium citrate, 2.0 g/L
bromocresol green, 0.04 g/L
captan, 0.004 g/L
dextrose, 20.0 g/L
dipotassium phosphate, 2.0 g/L
magnesium sulfate, 0.1 g/L
manganese sulfate, 0.05 g/L
2-phenylethyl alcohol, 3.0 g/L
proteose peptone (vegetable), 10.0 g/L
sodium acetate, 5.0 g/L
yeast extract, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.5±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
microbiology

suitability

selective and differential for Lactobacillus spp.
selective by low pH for Bifidobacterium spp.

Quality Level

200

Related Categories

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

MRS Agar, Vegitone has been used for culturing yogurt to analyze lactic acid bacteria (LAB). It has also been used in culturing of Lactobacillus reuteri colonies.

General description

This MRS Agar is free of animal derived material. It is recommended for the isolation and cultivation of Lactobacillus species.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 62.2 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN® 80; Cat. No. P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type N95 (US)

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN4982
BCCM7646
BCCM0516
BCCK3227
BCCJ9046

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He Ni et al.
Heliyon, 4(10), e00875-e00875 (2018-11-06)
In this study aqueous extracts from salal berry (SB) and blackcurrant pomace (BCP) were used to reformulate yogurt and the anti-diabetic properties of the beverage were investigated during 4 weeks of cold storage at 4 °C. Results indicated that α-amylase
Ferdinand Sedlmayer et al.
Nature communications, 9(1), 1822-1822 (2018-05-10)
Quorum sensing is a promising target for next-generation anti-infectives designed to address evolving bacterial drug resistance. The autoinducer-2 (AI-2) is a key quorum-sensing signal molecule which regulates bacterial group behaviors and is recognized by many Gram-negative and Gram-positive bacteria. Here

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Microbiological Media & Detection

Cost-effective molecular biology method for lactobacilli detection is presented as an alternative to PCR-based methods.

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