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319112

Sigma-Aldrich

Methylene Blue solution

0.05 wt. % in H2O

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Synonym(s):
Ehrlich’s reagent III, Loeffler’s Methylene Blue
Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
61-73-4
Molecular Weight:
319.85
Colour Index Number:
52015
Beilstein:
3641570
MDL number:
MFCD00012111
UNSPSC Code:
12171500
PubChem Substance ID:
24858888
NACRES:
NA.47

form

liquid

Quality Level

200

concentration

0.05 wt. % in H2O

density

0.98 g/mL at 25 °C

λmax

665 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

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Application

Methylene Blue solution has been used to study structural alterations and p53 immune-expression in rats.

Biochem/physiol Actions

Methylene blue (MB) is a highly recognized histological dye. It plays a vital role in microbiology and pharmacology. It has been employed in staining living organisms and in methemoglobinemia treatment. MB exhibits maximum absorption at 664nm. Exposure to methylene blue leads to various health hazards such as increased heart rate, vomiting, shock, Heinz body formation, cyanosis, jaundice, quadriplegia and tissue necrosis in humans

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Methylene blue in photodynamic therapy: From basic mechanisms to clinical applications.
Tardivo JP
Photodiagnosis and Photodynamic Therapy, 2, 175-191 (2005)
Microscopic structural alterations
and p53 immune-expression in adult
rat testis in response to intra-tunical versus
intra-parenchymal injection of methylene blue
Mazroa S A
International Journal of Clinical and Experimental Pathology, 9, 5346-5356 (2016)
J How et al.
Gynecologic oncology, 137(3), 436-442 (2015-04-15)
With the debate over extent of lymphadenectomy in endometrial cancer, sentinel lymph node (SLN) mapping may provide a focused approach to evaluate the most relevant lymph nodes (LN) while minimizing the complications. We evaluated SLN mapping using filtered technetium(99), indocyanine
Bram Vivijs et al.
Applied and environmental microbiology, 80(19), 6054-6061 (2014-07-27)
Some members of the family Enterobacteriaceae ferment sugars via the mixed-acid fermentation pathway. This yields large amounts of acids, causing strong and sometimes even lethal acidification of the environment. Other family members employ the 2,3-butanediol fermentation pathway, which generates comparatively
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