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319112

Sigma-Aldrich

Methylene Blue solution

0.05 wt. % in H2O

Synonym(s):

Ehrlich’s reagent III, Loeffler’s Methylene Blue

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250 REACTIONS
CN¥3,107.76
1250 REACTIONS
CN¥14,336.31

CN¥3,107.76

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250 REACTIONS
CN¥3,107.76
1250 REACTIONS
CN¥14,336.31

About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
61-73-4
Molecular Weight:
319.85
Colour Index Number:
52015
Beilstein:
3641570
MDL number:
MFCD00012111
UNSPSC Code:
12171500
PubChem Substance ID:
24858888
NACRES:
NA.47

CN¥3,107.76

Please contact Customer Service for Availability
New, lower price on this item!
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form

liquid

Quality Level

200

concentration

0.05 wt. % in H2O

density

0.98 g/mL at 25 °C

λmax

665 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

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KiCqStart® SYBR® Green qPCR ReadyMix™ with ROX™ for ABI instruments

KCQS02

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KCQS00

KiCqStart® SYBR® Green qPCR ReadyMix™

KiCqStart® SYBR® Green qPCR ReadyMix™ Low ROX™, for ABI and Stratagene instruments

KCQS01

KiCqStart® SYBR® Green qPCR ReadyMix™

KiCqStart® One-Step Probe RT-qPCR ReadyMix™ for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

KCQS07

KiCqStart® One-Step Probe RT-qPCR ReadyMix™

form

liquid

form

liquid

form

liquid

form

liquid

usage

sufficient for 1250 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

usage

sufficient for 500 reactions

feature

dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

storage condition

protect from light

storage condition

protect from light

storage condition

protect from light

storage condition

protect from light

color

colorless

color

colorless

color

colorless

color

light blue

Application

Methylene Blue solution has been used to study structural alterations and p53 immune-expression in rats.[1]

Biochem/physiol Actions

Methylene blue (MB) is a highly recognized histological dye. It plays a vital role in microbiology and pharmacology. It has been employed in staining living organisms and in methemoglobinemia treatment. MB exhibits maximum absorption at 664nm.[2] Exposure to methylene blue leads to various health hazards such as increased heart rate, vomiting, shock, Heinz body formation, cyanosis, jaundice, quadriplegia and tissue necrosis in humans

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SHBS4972
    SHBR7946
    MKCK4473
    MKCD3437
    MKBX9824V

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    Methylene blue in photodynamic therapy: From basic mechanisms to clinical applications.
    Tardivo JP
    Photodiagnosis and Photodynamic Therapy, 2, 175-191 (2005)
    Alexandru Grigoroiu et al.
    Scientific reports, 10(1), 3947-3947 (2020-03-05)
    Hyperspectral imaging (HSI) is being explored in endoscopy as a tool to extract biochemical information that may improve contrast for early cancer detection in the gastrointestinal tract. Motion artefacts during medical endoscopy have traditionally limited HSI application, however, recent developments
    Microscopic structural alterations
    and p53 immune-expression in adult
    rat testis in response to intra-tunical versus
    intra-parenchymal injection of methylene blue
    Mazroa S A
    International Journal of Clinical and Experimental Pathology, 9, 5346-5356 (2016)
    J How et al.
    Gynecologic oncology, 137(3), 436-442 (2015-04-15)
    With the debate over extent of lymphadenectomy in endometrial cancer, sentinel lymph node (SLN) mapping may provide a focused approach to evaluate the most relevant lymph nodes (LN) while minimizing the complications. We evaluated SLN mapping using filtered technetium(99), indocyanine
    Bram Vivijs et al.
    Applied and environmental microbiology, 80(19), 6054-6061 (2014-07-27)
    Some members of the family Enterobacteriaceae ferment sugars via the mixed-acid fermentation pathway. This yields large amounts of acids, causing strong and sometimes even lethal acidification of the environment. Other family members employ the 2,3-butanediol fermentation pathway, which generates comparatively
    View All Related Papers

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