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Merck
CN

199664

Sudan Black B

certified by the BSC

Synonym(s):

Ceres black BN, Fat Black HB, Solvent Black 3

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About This Item

Empirical Formula (Hill Notation):
C29H24N6
CAS Number:
4197-25-5
Molecular Weight:
456.54
Colour Index Number:
26150
Beilstein:
723248
EC Number:
224-087-1
MDL number:
MFCD00006919
UNSPSC Code:
12171500
PubChem Substance ID:
24851932
NACRES:
NA.47

Key Documents

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Agency

certified by the BSC

Quality Level

200

form

powder

technique(s)

microbe id | staining: suitable

color

dark brown to very dark brown

mp

120-124 °C (lit.)

λmax

415 nm (2nd)
598 nm

ε (extinction coefficient)

≥20000 at 596-603 nm in ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46

InChI

1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+

InChI key

YCUVUDODLRLVIC-VPHDGDOJSA-N

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Application

Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.
Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.

Biochem/physiol Actions

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H341

Hazard Classifications

Muta. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS3884
SHBQ4629
SHBR1166
SHBQ0335
SHBN2602

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Brain BLAQ: Post-hoc thick-section histochemistry for localizing optogenetic constructs in neurons and their distal terminals
Kupferschmidt DA
Frontiers in Neuroanatomy (2015)
H N Subramaniam et al.
Journal of biochemical and biophysical methods, 21(1), 9-16 (1990-06-01)
The selective staining of neutral lipids in Human Amnion cells in tissue culture was achieved using a particular fraction of the lipid dye, Sudan black B and a standardized staining procedure. The fraction, termed SBB-I, was isolated by thin-layer chromatography.
Acetylated Sudan Black B as a More Specific Histochemical Reagent for Lipides
CASSELMAN WGB
Journal of Cell Science, 321- 322 (1954)
John P. Greer
Wintrobe's Clinical Hematology, 1 (2009)
Effects of feeding different lipid sources on hepatic histopathology features and growth traits of broiler chickens
Tufarelli V, et.al.
Acta Histochemica, 117, 780-783 (2015)
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