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199664

Sigma-Aldrich

Sudan Black B

certified by the BSC

Synonym(s):

Ceres black BN, Fat Black HB, Solvent Black 3

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25 G
CN¥966.72

CN¥966.72

List PriceCN¥1,092.39Save 12%

Available to ship onApril 16, 2025Details

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25 G
CN¥966.72

About This Item

Empirical Formula (Hill Notation):
C29H24N6
CAS Number:
4197-25-5
Molecular Weight:
456.54
Colour Index Number:
26150
Beilstein:
723248
EC Number:
224-087-1
MDL number:
MFCD00006919
UNSPSC Code:
12171500
PubChem Substance ID:
24851932
NACRES:
NA.47

CN¥966.72

List PriceCN¥1,092.39Save 12%

Available to ship onApril 16, 2025Details

Request a Bulk Order

Agency

certified by the BSC

Quality Level

200

form

powder

technique(s)

microbe id | staining: suitable

color

dark brown to very dark brown

mp

120-124 °C (lit.)

λmax

415 nm (2nd)
598 nm

ε (extinction coefficient)

≥20000 at 596-603 nm in ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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1 of 4

This Item
211842S4261S4136
Sudan Black B certified by the BSC

199664

Sudan Black B

Brilliant Black BN Dye content 60 %

211842

Brilliant Black BN

Sudan IV BioXtra, certified by the BSC

S4261

Sudan IV

Sudan III certified by the BSC, BioXtra

S4136

Sudan III

color

dark brown to very dark brown

color

-

color

-

color

-

form

powder

form

powder

form

powder

form

powder

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

ε (extinction coefficient)

≥20000 at 596-603 nm in ethanol

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Application

Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.[1][2]

Biochem/physiol Actions

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time.[3] It is considered to be a most sensitive and specific reagent for lipid staining.[4] Sudan Black B stains phospholipids gray and neutral fats blue-black.[5]

Suitability

Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H341

Hazard Classifications

Muta. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SHBS3884
    SHBQ4629
    SHBR1166
    SHBQ0335
    SHBN2602

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Effects of feeding different lipid sources on hepatic histopathology features and growth traits of broiler chickens
    Tufarelli V, et.al.
    Acta Histochemica, 117, 780-783 (2015)
    H N Subramaniam et al.
    Journal of biochemical and biophysical methods, 21(1), 9-16 (1990-06-01)
    The selective staining of neutral lipids in Human Amnion cells in tissue culture was achieved using a particular fraction of the lipid dye, Sudan black B and a standardized staining procedure. The fraction, termed SBB-I, was isolated by thin-layer chromatography.
    Acetylated Sudan Black B as a More Specific Histochemical Reagent for Lipides
    CASSELMAN WGB
    Journal of Cell Science, 321- 322 (1954)
    Brain BLAQ: Post-hoc thick-section histochemistry for localizing optogenetic constructs in neurons and their distal terminals
    Kupferschmidt DA
    Frontiers in Neuroanatomy (2015)
    Vincenzo Tufarelli et al.
    Acta histochemica, 117(8), 780-783 (2015-08-19)
    The effects of different dietary lipid sources on growth traits and hepatic histopathology of broiler chickens were investigated. Hubbard strain one-day old chickens (n=120) were kept in pens and were fed one of the three corn-soybean meal-based diets until 49
    View All Related Papers

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