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Merck
CN

17215

WL Differential Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):

WLD Agar, Wallerstein Differential Agar

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171606

Key Documents

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Product Name

WL Differential Agar, suitable for microbiology, NutriSelect® Plus

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

actidione (cycloheximide), 0.004 g/L
agar, 20 g/L
bromocresol green, 0.022 g/L
calcium chloride, 0.125 g/L
casein enzymic hydrolysate, 5 g/L
dextrose, 50 g/L
ferric chloride, 0.0025 g/L
magnesium sulfate, 0.125 g/L
manganese sulfate, 0.0025 g/L
monopotassium phosphate, 0.55 g/L
potassium chloride, 0.425 g/L
yeast extract, 4 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.5±0.2 (25 °C)

application(s)

bioburden testing
food and beverages
microbiology

suitability

selective and differential for Escherichia coli
selective and differential for Lactobacillus spp.
selective and differential for Proteus spp.
selective and differential for bacteria (General Media)
selective and differential for coliforms

Quality Level

200

Related Categories

Application

WL Differential Agar has been used for the enumeration of Kluyveromyces marxianus culture.

General description

WL (Wallerstein Laboratory) Differential Agar, modified is formulated as described by Green and Gray for the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeasts and bacteria. WL Differential Agar helps in selective isolation and enumeration of bacteria encountered in breweries and industrial fermentations.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 80.26 g in 1 litre distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. If desired, to obtain a pH of 6.5, add 1% solution of sodium bicarbonate.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN3875
BCCM7669
BCCL8851
BCCK4928
BCCH1945

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Interactions between Kluyveromyces marxianus and Saccharomyces cerevisiae in tequila must type medium fermentation
Lopez CLF, et al.
World Journal of Microbiology & Biotechnology, 30(8), 2223-2229 (2014)
A differential procedure for bacteriological studies useful in the fermentation industry
Green SR and Gray PP
Archives of Biochemistry and Biophysics, 32(1), 59-69 (1951)

Articles

Culture Method for Beer Quality Control

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Microbiological Media & Detection

Cost-effective molecular biology method for lactobacilli detection is presented as an alternative to PCR-based methods.

Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

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