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Safety Information

RDLIG-RO

Roche

Rapid DNA Dephos & Ligation Kit

sufficient for 40 reactions (04898117001), sufficient for 160 reactions (04898125001), optimum reaction temp. 37 °C

Synonym(s):

cloning of dna fragments, dna ligase, ligases

Sign Into View Organizational & Contract Pricing

Select a Size

1 L
CN¥563.70
4 L
CN¥2,215.25
10 L
CN¥5,273.66

CN¥563.70

Please contact Customer Service for Availability
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1 L
CN¥563.70
4 L
CN¥2,215.25
10 L
CN¥5,273.66

About This Item

Enzyme Commission number:
3.1.3.1 (BRENDA, IUBMB)
UNSPSC Code:
12352200
NACRES:
NA.55

CN¥563.70

Please contact Customer Service for Availability

usage

sufficient for 160 reactions (04898125001)
 sufficient for 40 reactions (04898117001)

Quality Level

100

manufacturer/tradename

Roche

parameter

37 °C optimum reaction temp.

storage temp.

−20°C

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technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

application(s)

environmental

application(s)

environmental

application(s)

environmental

application(s)

environmental

format

neat

format

neat

format

single component solution

format

single component solution

grade

analytical standard

grade

certified reference material

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

storage temp.

2-30°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

packaging

ampule of 100 mg

packaging

-

packaging

ampule of 5 × 1 mL

packaging

ampule of 1 mL

General description

The Rapid DNA Dephos & Ligation Kit enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments.

Specificity

Specificity: rAPid Alkaline Phosphatase
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.

Application

The Rapid DNA Dephos & Ligation Kit minimizes the time needed to perform diverse applications, including:
  • Cloning of fragments into either plasmid or phage vectors[1]
  • Linker ligation
  • Recircularization of linear vector DNA
  • Generation of libraries

Packaging

1 kit containing 5 components

Unit Definition

Unit Definition: rAPid Alkaline Phosphatase
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one μmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.

Volume Activity: rApid Alkaline Phosphatase: 1 U/μl
T4 DNA Ligase: 5 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description
  • rAPid Alkaline Phosphatase Buffer 10x concentrated
  • rAPid Alkaline Phosphatase 1 U/μl
  • DNA Dilution Buffer 5x concentrated
  • T4 DNA Ligation Buffer 2x concentrated
  • T4 DNA Ligase 5 U/μl

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

常规特殊物品
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    52250800
    50235900
    49909400
    42989000
    39466000

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    Francesca Colombo et al.
    Molecular cancer, 10, 81-81 (2011-07-09)
    The MFSD2A gene maps within a linkage disequilibrium block containing the MYCL1-EcoRI polymorphism associated with prognosis and survival in lung cancer patients. Survival discrepancies between Asians and Caucasians point to ethnic differences in allelic frequencies of the functional genetic variations.

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