G418-RO
Roche
G-418 Solution
solution, =98% (TLC), suitable for transfection
Synonym(s):
G 418 disulfate salt solution, O-2-Amino-2,7-dideoxy-Δ-glycero-α-Δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-Δ-streptamine, disulfate salt, neomycin, Antibiotic G418
About This Item
Quality Level
Assay
98% (TLC)
form
solution
mol wt
692.7 Da
packaging
pkg of 100 ml (04727894001 [5 x 20 ml, equivalent to 5 g])
pkg of 20 mL (04727878001 [equivalent to 1 g])
manufacturer/tradename
Roche
concentration
50 mg/mL
technique(s)
transfection: suitable
antibiotic activity spectrum
protozoa
Mode of action
protein synthesis | inhibits
storage temp.
2-8°C
SMILES string
OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
InChI
1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
InChI key
UHEPSJJJMTWUCP-NKCAIAFTSA-N
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General description
Application
G-418 is also used to eliminate contaminating fibroblasts from mixed cultures.
Biochem/physiol Actions
Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.
Features and Benefits
- Convenient sterile-filtered solution for immediate use – saves valuable time and effort, and avoids potential errors arising from the preparation of sterile working stock solution from G-418 powder.
- Consistent quality and potency – shows no lot-to-lot variation in its ability to select resistant cells.
- Function tested in a cell culture assay - ensures effectiveness.
- Very pure, as indicated by TLC and microbiological assays – allows you to use less antibiotic while producing greater selection pressure. Lack of contaminants means minimal toxicity.
- Cost effective, since the selected colonies are stable.
Aqueous solution of G-418, 892 μg/mg, filtered through 0.2μm pore size membrane
Quality
Function testing: in cell culture.
Preparation Note
Active weight: >700μg/mg
Recommended concentration in cell culture: The optimal concentration of G-418 must be determined experimentally; it varies from 100μg/ml to 1mg/ml, depending on the cell type used.
Specific rotation: 104° to 121°, in water (on dry basis, according to USP XXII).
Other Notes
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
No data available
Flash Point(C)
No data available
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
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