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11088939001

Roche

Tumor Necrosis Factor-α, human (hTNF-α)

recombinant (yeast)

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Synonym(s):
hTNF, human necrosis factor
UNSPSC Code:
12352207

biological source

human

Quality Level

100

recombinant

expressed in yeast

Assay

≥95% (SDS-PAGE)

form

solution

potency

≤0.01 ng/mL EC50

specific activity

>1*10^8 U/mg (>1 x 10⊥8 U/mg, <0.01 ng/ml (hTNF-a, NIBSC, interim standard, 87/650), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.)

mol wt

17,000 Da

packaging

pkg of 1,000,000 U (10 μg, 1ml)

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

impurities

<0.1 EU/μgtested (LAL test)

UniProt accession no.

Q9UBM5

storage temp.

−20°C

Gene Information

human ... TNF(7124)

Related Categories

General description

Tumor necrosis factor a (TNF-a) is a multifunctional cytokine, majorly produced by activated macrophages and is involved in the pathogenesis of many acute and chronic inflammatory condition.

Recombinant, Tumor Necrosis Factor-α, human (hTNF-α), is produced in Pichia pastoris and purified by standard chromatographic techniques.

Primary structure: One polypeptide chain (157 amino acids), identical to natural hTNF-a.

Specificity

Human hTNF-α is effective on human and mouse cells.
Species specificity: human, mouse

Application

Tumor Necrosis Factor-α (hTNF-α)
  • Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines.
  • Has activating and growth stimulating activities on a variety of normal cells.
  • Has antiviral activity on many cell types in vitro.
  • Can cause tumor necrosis of certain tumors in vivo.

Biochem/physiol Actions

Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.

Quality

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

Sequence

Chain Length 157 AA
One polypeptide chain (157 amino acids), identical to natural hTNF-α.

Unit Definition

EC50 definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml).

Physical form

Solution, filtered through 0.2 μm pore size membrane.

Preparation Note

Working concentration: 1 ng/ml
For complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.
Working solution: Dilute the concentrated hTNF-α solution (10 μg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.
Storage conditions (working solution): -15 to -25 °C
It is recommended to store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Other Notes

For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

含少量动物源组分生物产品
常规特殊物品

Certificates of Analysis (COA)

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P De Cesaris et al.
The Journal of biological chemistry, 274(41), 28978-28982 (1999-10-03)
Tumor necrosis factor alpha (TNF-alpha) is a cytokine implicated in the pathogenesis of numerous chronic and acute inflammatory conditions. We have previously shown that mouse Sertoli cells respond to TNF-alpha by increasing interleukin-6 production and intercellular adhesion molecule-1 (ICAM-1) expression
Rajitha Indukuri et al.
Frontiers in endocrinology, 12, 650625-650625 (2021-04-17)
Inflammation is a primary component of both initiation and promotion of colorectal cancer (CRC). Cytokines secreted by macrophages, including tumor necrosis factor alpha (TNFα), activates the pro-survival transcription factor complex NFκB. The precise mechanism of NFκB in CRC is not

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