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10814407001

Roche

Peroxidase (POD)

EIA grade, from horseradish

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Synonym(s):
POD
Enzyme Commission number:
1.11.1.7 (BRENDA, IUBMB)
UNSPSC Code:
12352204

biological source

horseradish

Quality Level

100

Assay

90% (HPLC)

form

lyophilized (salt-free)

specific activity

~225 U/mg lyophilizate, 25 °C (Guaiacol, H2O2)

packaging

pkg of 100 mg

manufacturer/tradename

Roche

concentration

10 g/L

technique(s)

activity assay: suitable

color

red to brown

optimum pH

6.0-6.5

solubility

water: soluble at 68 °F

suitability

suitable for molecular biology

UniProt accession no.

P00433

application(s)

detection
life science and biopharma

foreign activity

ATPase <0.00100%, none detected
AcP <0.00100%
Catalase <0.700%

shipped in

dry ice

storage temp.

−20°C (−15°C to −25°C)

Related Categories

General description

Donor: hydrogen-peroxide oxidoreductase
Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.

Application

Peroxidase (POD) has been used in the spermine oxidase enzyme activity assay.

Sequence

Blocking substances
Preparations does not contain substances that block the amino groups, but in contrast to other enzymes POD has only 2 to 3 free amino groups which are not accessible; even by extended dialysis of POD, the number of NH2-groups/mol does not change.

POD
POD contains 1 molecule of protoheme IX as prosthetic group and 2 molecules of Ca2+.
POD exists in > 10 isoenzymic forms (some sources estimate approx. 40 isoenzymes).

Carbohydrate Content
12 to 14.5 mg/100 mg Iyophilizate

Free Amino Groups
Free amino groups: 2 to 3 mol/mol enzyme

Preparation Note

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents
Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).
Specific activity: approximately 225U/mg lyophilizate (+25°C, guaiacol, H2O2) approximately 1,000 U/mg lyophilizate (+25°C, ABTS, H2O2, pH 5.0) approximately 3,500 U/mg lyophilizate (+25°C, tetramethylbenzidine, H2O2)
Amino groups: 2 - 3moles/mole enzyme
Carbohydrates: 12.0 - 14.5% (w/w)
Isoenzymes: ≥90% isoenzyme C (HPLC)
Purity: single peak (HPLC)
Purity number: 3.0 - 3.5 (A403/A275)

Storage and Stability

Keep container tightly closed in a dry and well-ventilated place.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Andrew C Goodwin et al.
Methods in molecular biology (Clifton, N.J.), 720, 173-181 (2011-02-15)
Spermine oxidase (SMO), the most recently characterized polyamine metabolic enzyme, catalyzes the direct back-conversion of spermine to spermidine in an FAD-dependent reaction that also yields the byproducts hydrogen peroxide (H(2)O(2)) and 3-aminopropanal. These metabolites, particularly H(2)O(2), have been implicated in

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Horseradish Peroxidase (HRP) Enzymes

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