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SCR128

Sigma-Aldrich

Dopaminergic Differentiation Growth Factor Sampler

This Dopaminergic Differentiation Growth Factor Sampler contains five validated growth factors used to induce differentiation of human pluripotent Embryonic stem (ES) & induced pluripotent Stem (iPS) cells to dopaminergic neurons.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

100

Assay

>98% (SDS-PAGE and HPLC)

impurities

<0.1 ng/μg endotoxin (1EU/μg)

shipped in

dry ice

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separation technique

hydrophilic interaction (HILIC), supercritical fluid (SFC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

particle size

5 μm

particle size

5 μm

particle size

5 μm

particle size

5 μm

matrix

fully porous particle

matrix

fully porous particle

matrix

fully porous particle

matrix

fully porous particle

product line

Purospher®

product line

Purospher®

product line

Purospher®

product line

Purospher®

pore size

120 Å pore size

pore size

120 Å pore size

pore size

120 Å pore size

pore size

120 Å pore size

General description

EMD Millipore’s Dopaminergic Differentiation Growth Factor Sampler contains a collection of five validated growth factors that are routinely used to induce differentiation of human pluripotent ES/iPS cells to dopaminergic neurons along with a reliable high affinity antibody marker, anti-tyrosine hydrodylase, to aid in quantifying the percentage yields from differentiation experiments.
Product Source: E. coli.

Specificity

Cross Reactivty
None

Application

Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
This Dopaminergic Differentiation Growth Factor Sampler contains five validated growth factors used to induce differentiation of human pluripotent Embryonic stem (ES) & induced pluripotent Stem (iPS) cells to dopaminergic neurons.

Components

1. Sonic HedgeHog (Shh), Human Recombinant (Part No. GF174-5UG): Two 5 μg vials. Lyophilized powder. Store at -20°C.

2. Fibroblast Growth Factor-8 (FGF-8), Human Recombinant (Part No. GF110-5UG): One 5 μg vial. Lyophilized powder. Store at -20°C.

3. Brain Derived Neurotrophic Factor (BDNF), Human Recombinant (Part No. GF029-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.

4. Glial Derived Neurotrophic Factor (GDNF), Human Recombinant (Part No. GF030-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.

5. Transforming Growth Factor β-III (TGF-β-III), Human Recombinant (Part No. GF176-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.

6. Anti-Tyrosine Hydroxylase antibody, rabbit polyclonal (Part No. AB152-20): One 20 μL vial. Store at -20°C.

Quality

Using the growth factors supplied in this kit a 3-5 fold increase of dopaminergic differention (up to 50-70% TH+ cells) was routinely produced using HES/hips cell derived neural progenitor cells vs. spontaneous neuronal differentiation.

Storage and Stability

Store at -20°C for up to 6 months from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

Regulatory Information

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Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.

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