PC35
Anti-p53 (Ab-7) (Pantropic) Sheep pAb
liquid, Calbiochem®
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
biological source
sheep
Quality Level
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative (Anti-p53 and Anti-Sheep IgG only)
species reactivity
human, rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TP53(7157)
General description
Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 and SVT2 cells and breast carcinoma tissue.
Sheep polyclonal antibody supplied as diluted serum. Recognizes the ~53 kDa wild-type or mutant p53 protein. Supplied with normal sheep serum and biotinylated rabbit anti-sheep IgG.
This Anti-p53 (Ab-7) (Pantropic) Sheep pAb is validated for use in Frozen Sections, Immunoblotting, ICC, Paraffin Sections, IP for the detection of p53 (Ab-7) (Pantropic).
Immunogen
Human
recombinant, human p53 protein
Application
Frozen Sections (1:500)
Immunoblotting (1:2500)
Immunocytochemistry (1:500)
Paraffin Sections (1:500, pepsin or heat pre-treatment required)
Immunoprecipitation (chromatin, see application references)
Components
Anti-p53 antisera, normal sheep serum, and biotinylated rabbit anti-sheep IgG
Warning
Toxicity: Standard Handling (A)
Physical form
200 µl diluted (1:4) anti-p53 sheep serum, 500 µl undiluted normal sheep serum, and 100 µl biotinylated rabbit anti-sheep IgG.
Analysis Note
Positive Control
A431 or SVT2 cells or breast carcinoma tissue
A431 or SVT2 cells or breast carcinoma tissue
Other Notes
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.
Recommended dilutions for the biotinylated rabbit anti-sheep secondary are 1:25,000-1:200,000 for immunoblotting and 1:10,000 for immunocytochemistry and paraffin sections. For immunoblotting, do not add normal serum, non-fat dried milk, culture medium or other potential sources of biotin to the working solutions (i.e. block with 2-5% fatty acid free BSA). Use streptavidin-horseradish peroxidase (Cat. No. OR03L) or streptavidin-alkaline phosphatase (Cat. No. OR04L) at 10 ng/ml to attach an enzyme to the biotinylated secondary antibody, then visualize using standard colorimetric or chemiluminescent reagents. To maximize sensitivity of immunoblots, preconcentrate samples by immunoprecipitation with Cat. No. OP03, OP09, or OP43, then immunoblot using Cat. No. PC35 and chemiluminescent detection. Antibodies should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Vashe Chandrakanthan et al.
Reproductive biology and endocrinology : RB&E, 4, 61-61 (2006-11-23)
In the mouse, embryo culture results in a characteristic phenotype of retarded embryo preimplantation development and reduced numbers of cells within embryos. The expression of TRP53 is central to the regulation of the cell's capacity to proliferate and survive. In
Kenneth A Giuliano
Methods in molecular biology (Clifton, N.J.), 356, 189-193 (2006-09-22)
Immunoreagents formed the basis of early fixed end point high content screening (HCS) assays and their use in HCS applications in drug discovery will continue to increase. One important application of immunoreagents is their incorporation into multiplexed HCS assays in
Joshua D Dowell et al.
Biochimica et biophysica acta, 1773(3), 358-366 (2007-01-19)
p193/CUL7 is an E3 ubiquitin ligase initially identified as an SV40 Large T Antigen binding protein. Expression of a dominant interfering variant of mouse p193/CUL7 (designated 1152stop) conferred resistance to MG132- and etoposide-induced apoptosis in U2OS cells. Immune precipitation/Western analyses
M Ciciarello et al.
The Journal of biological chemistry, 276(22), 19205-19213 (2001-05-29)
Growing evidence indicates a central role for p53 in mediating cell cycle arrest in response to mitotic spindle defects so as to prevent rereplication in cells in which the mitotic division has failed. Here we report that a transient inhibition
A Li et al.
Biology of reproduction, 76(3), 362-367 (2006-11-10)
The expression of TRP53 in blastocysts that had been cultured from the zygote stage in vitro for 90 h was compared with that in blastocysts collected from the uterus in C57BL6 (B6) and in F1 hybrid (B6CBF1) strain mice. In
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service