MABT538
Anti-Lamin A/C Antibody, clone 2E8.2
clone 2E8.2, from mouse
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Synonym(s):
Prelamin-A/C, Lamin-A/C, Renal carcinoma antigen NY-REN-32
UNSPSC Code:
12352203
eCl@ss:
32160702
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biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
2E8.2, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... LMNA(4000)
General description
Lamin-A is a key component of the nuclear lamina, a protein network underlying the inner membrane that determines nuclear shape and size. The same gene (Lmna, Dhe, or Lamn1 in murine species; Gene ID 16905) that encodes lamin-A also codes for the alternatively spliced prelamin-C isoforms (UniProt P48678-2 and P48678-3). Murine lamin-A (UniProt P48678-1) is initially produced as a 665-amino acid prelamin A, which is further processed posttranslationally by four enzymatic steps to yield the mature Lamin-A. Farnesylation of Cys662, endoproteolytic cleavage of the last three amino acids (a.a. 663-665), carboxyl methylation of Cys662, and the final endoproteolytic removal of the remaining propeptide sequence (a.a. 648-662), including the farnesylated Cys662, catalyzed by the ER membrane zinc metalloproteinase ZMPSTE24.
Specificity
Clone 2E8.2 immunostained nuclear membrane in human tissue sections.
Immunogen
GST-tagged recombinant fragment within the C-terminal half of human Lamin A.
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot immunostained nuclear membrane in human cerebellum, kidney, and pancreas tissue sections.
Research Category
Cell Structure
Cell Structure
This mouse monoclonal Anti-Lamin A/C Antibody, clone 2E8.2, Cat. No. MABT538 is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of lamin A/C.
Quality
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected lamin A/C in 10 µg of A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected lamin A/C in 10 µg of A431 cell lysate.
Target description
~70/65 kDa observed. 74.14/73.81/72.22 kDa (perpro-/pre-/mature Lamin A) and 65.14 kDa (Lamin C) calculated. Uncharacterized bands may be observed in some lysate(s).
Linkage
Replaces: MAB3211
Physical form
Format: Purified
Protein G purified.
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
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Luke W Koblan et al.
Nature, 589(7843), 608-614 (2021-01-08)
Hutchinson-Gilford progeria syndrome (HGPS or progeria) is typically caused by a dominant-negative C•G-to-T•A mutation (c.1824 C>T; p.G608G) in LMNA, the gene that encodes nuclear lamin A. This mutation causes RNA mis-splicing that produces progerin, a toxic protein that induces rapid
Zhuo Zhou et al.
Signal transduction and targeted therapy, 6(1), 382-382 (2021-11-05)
The global coronavirus disease 2019 (COVID-19) pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a positive-sense RNA virus. How the host immune system senses and responds to SARS-CoV-2 infection remain largely unresolved. Here, we report that SARS-CoV-2
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