MABT522
Anti-alpha-Tubulin Antibody, clone RM113
clone RM113, from rabbit
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Synonym(s):
Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, alpha-Tubulin
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
RM113, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TUBA1A(7846)
General description
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Immunogen
A peptide corresponding to the C-terminus of α-Tubulin.
Application
Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected alpha-Tubulin in A431 cells.
This Anti-alpha-Tubulin Antibody, clone RM113 is validated for use in Western Blotting, Immunocytochemistry for the detection of alpha-Tubulin.
Quality
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected alpha-Tubulin in A431 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected alpha-Tubulin in A431 cell lysate.
Target description
~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Wei Yu et al.
Frontiers in cell and developmental biology, 10, 918235-918235 (2023-01-03)
SATB homeobox proteins are important regulators of developmental gene expression. Among the stem cell lineages that emerge during early embryonic development, trophoblast stem (TS) cells exhibit robust SATB expression. Both SATB1 and SATB2 act to maintain the trophoblast stem-state. However
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