Anti-Utrophin Antibody, clone MANCHO3 (8A4)

Utrophin (UniProt: P46939; also known as Dystrophin-related protein 1, DRP-1) is encoded by the UTRN (also known as DMDL, DRP1) gene (Gene ID: 7402) in human. Utrophin is a dystrophin related protein that displays a sequence homology with dystrophin and shares many of the protein-binding properties. It plays a role in anchoring the cytoskeleton to the plasma membrane. It contains 2 calponin homology domains in its N-terminal region (aa 31-135 and 150-255) and has 22 spectrin homology repeats. The C-terminal sequence in utrophin is highly conserved between dystrophin and utrophin in most species. Utrophin is distributed throughout the sarcolemma in fetal and regenerating muscle, but is shown to be down-regulated in normal adult muscle and is restricted to the myotendinous and neuromuscular junctions. Four different isoforms of utrophin have been described that are produced by alternative promoter usage and they differ in their distribution pattern. Isoform 1 displays high expression in muscle tissue. Similar to dystrophin, utrophin can also reported to form an extensive lateral association with actin filaments and protect actin filaments from depolymerization in vitro. However, utrophin binds laterally along actin filaments through contribution of acidic spectrin-like repeats rather than the cluster of basic repeats used by dystrophin. It has been suggested that utrophin could functionally compensate for the lack of dystrophin and studied have shown that transgenic overexpression of utrophin in dystrophin-deficient mdx mice results in full recovery for all known parameters of the dystrophic phenotype. (Ref.: Sengupta, K., et al. (2020). Sci. Rep. 10; 21492; Porter, JD., et al.(1998). J. Cell Sci. 111(13); 1801-1811; Rybakova, IN., et al., (2002). Mol. Biol. Cell. 13(5); 1512-1521).

Clone MANCHO3 (8A4) is a mouse monoclonal antibody that detects Utrophin. It targets an epitope within 42 amino acids from the C-terminal region.

A recombinant fragment corresponding the 329 amino acids from the C-terminal region of human Utrophin.

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Utrophin in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Utrophin in NIH3T3 cell lysate.

Immunofluorescence Analysis: A representative lot detected Utrophin in Immunofluorescence applications (McMorran, B.J., et al. (2016). Glycobiology. 26(10):1120-1132).

Immunohistochemistry Applications: A representative lot detected Utrophin in Immunohistochemistry applications (Nguyen T.M. et al. (1991). J Cell Biol. 115(6):1695-700).

Western Blotting Analysis: A representative lot detected Utrophin in Western Blotting applications (Morris, G.E., et al. (1998). Biochemistry. 37(31):11117-27; Nguyen T.M. et al. (1991). J Cell Biol. 115(6):1695-700; Lien, C.F., et al. (2012). J Biol Chem. 287(49):41374-85; Ghosh, T., et al. (2015). PLoS One;10(7):e0134809; Lindsay, A., et al. (2019). FEBS J.;286(13):2562-2576).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Utrophin, clone MANCHO3 (8A4), Cat. No. MABT1558, is a mouse monoclonal antibody that detects Utrophin and is tested for use in Immunofluorescence, , Immunohistochemistry, and Western Blotting.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.