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MABF850

Sigma-Aldrich

Anti-IDO-1 Antibody, clone 4B7 | MABF850

clone 4B7, from mouse

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Synonym(s):
Indoleamine 2,3-dioxygenase 1, IDO-1, Indoleamine-pyrrole 2,3-dioxygenase
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4B7, monoclonal

species reactivity

mouse, rat

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_032350

UniProt accession no.

P28776

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Ido1(15930)

General description

Indoleamine 2,3-dioxygenase 1 (EC 1.13.11.52; UniProt P28776; also known as IDO-1, indole 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase) is encoded by the Ido1 (also known as Ido, Indo) gene (Gene ID 15930) in murine species. Indoleamine 2,3-dioxygenase-1 (IDO1) is a heme containing enzyme that catalyzes the rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. The reduction in local tryptophan and the upregulated immunomodulatory tryptophan metabolites form the basis of IDO-dependent regulation of inflammation and immunity. IDO is reported to support carcinogenesis by modulating tissue microenvironment inflammatory responses. IDO is also reported to mediate immune escape in cancer by suppressing the function of T effector cells and promote the differentiation of T regulatory cells.

Specificity

Target specificity of clone 4B7 has been confirmed by both immunohistochemistry and Western blotting using tissue samples from wild-type and Ido1-knockout mice (Thomas, S., et al. (2014). J. Cell. Biochem. 115(2):391-396).

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminal region of mouse IDO-1.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected IDO-1 in rat kidney tissue.
Immunohistochemistry Analysis: A representative lot detected IDO-1 immunoreactivity in acetone-fixed frozen colon tissue sections from wild-type, but not Ido1-knockout mice (Courtesy of Dr. Sunil Thomas, Lankenau Institute for Medical Research, Wynnewood, PA).
Immunofluorescence Analysis: A representative lot detected IDO-1 immunoreactivity in acetone-fixed frozen colon and epididymis tissue sections from wild-type, but not Ido1-knockout mice by fluorescent immunohistochemistry (Courtesy of Dr. Sunil Thomas, Lankenau Institute for Medical Research, Wynnewood, PA).
Western Blotting Analysis: A representative lot detected IDO-1 in epididymis and colon tissue extracts from wild-type, but not Ido1-knockout mice (Thomas, S., et al. (2014). J. Cell. Biochem. 115(2):391-396).
Immunohistochemistry Analysis: A representative lot detected IDO-1 immunoreactivity in multiple acetone-fixed frozen tissue sections from wild-type, but not Ido1-knockout mice, including epididymis, colon, heart, liver, and 4T1-metastasized lung tissue sections (Thomas, S., et al. (2014). J. Cell. Biochem. 115(2):391-396).
This Anti-IDO-1 Antibody, clone 4B7 | MABF850 is validated for use in Immunohistochemistry (Paraffin), Western Blotting for the detection of IDO-1.

Quality

Evaluated by Immunohistochemistry in mouse kidney tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected IDO-1 in mouse kidney tissue.

Target description

~45 kDa calculated

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yahya Jand et al.
Scientific reports, 12(1), 15963-15963 (2022-09-25)
Melatonin (MT), a neurohormone with immunomodulatory properties, is one of the metabolites produced in the brain from tryptophan (TRP) that has already strong links with the neuropathogenesis of Multiple sclerosis (MS). However, the exact molecular mechanisms behind that are not
Souvik Dey et al.
Journal for immunotherapy of cancer, 8(2) (2020-07-22)
The tryptophan-catabolizing enzyme indoleamine 2,3-dioxygenase 1 (IDO1), which subverts T-cell immunity at multiple levels, is itself subject to inherent T-cell reactivity. This intriguing deviation from central tolerance has been interpreted as counterbalancing IDO1-mediated immunosuppression. Based on this hypothesis, clinical studies
Willy Jaya Suento et al.
Journal of neurochemistry, 157(6), 1963-1978 (2020-10-24)
Indoleamine 2,3-dioxygenase 1 (IDO1) is the first rate-limiting enzyme that metabolizes tryptophan to the kynurenine pathway. Its activity is highly inducible by pro-inflammatory cytokines and correlates with the severity of major depressive disorder (MDD). MicroRNAs (miRNAs) are involved in gene
Tatsuya Ando et al.
Molecular medicine reports, 27(2) (2022-12-10)
The partial loss of liver due to liver transplantation or acute liver failure induces rapid liver regeneration. Recently, we reported that the selective inhibition of indoleamine 2,3‑dioxygenase (Ido) 1 promotes early liver regeneration. However, the role of Ido2 in liver
Yuying Liu et al.
Nature communications, 8, 15207-15207 (2017-05-11)
Interactions with the immune system may lead tumorigenic cells into dormancy. However, the underlying molecular mechanism is poorly understood. Using a 3D fibrin gel model, we show that IFN-γ induces tumour-repopulating cells (TRCs) to enter dormancy through an indolamine 2,3-dioxygenase
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