Anti-Complement C3a/C3a (desArg) Antibody, clone K13/16

Complement C3 (UniProt: P01024; also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1) is encoded by the C3 (also known as CPAMD1) gene (Gene ID: 718) in human. C3 is a secreted protein that plays a key role in the activation of the complement system. It s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. C3 has an anaphylatoxon-like domain (aa 693-728) and a NTR (Netrin) domain (aa 1518-1661). IC3 precursor is first processed by the removal of four Arginine residues, forming two chains, beta and alpha, linked by a disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylatoxin and generating C3b. C3a appears to be important in many inflammatory respons-es and the C3b fragment covalently binds to the cell or bacterial surface and plays a role in opsonisation. C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, it acts as a chemoattractant for neutrophils. Defects in C3 gene can cause complement component 3 deficiency that leads to recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Defects in C3 gene also cause age-related macular degeneration and hemolytic uremic syndrome that can lead to hemolytic anemia and renal failure

Clone K13/16 recognizes complement C3a in human tissues. It targets an epitope that is present on human C3, C3a, and C3a-desArg.

A full length native C3a fragment purified from human serum that was activated at 37°C for 1 hour by treatment with 10 mg/mL zymosan.

Anti-Complement C3a/C3a (desArg), clone K13/16, Cat. No. MABF1978, is a mouse monoclonal antibody that detects Complement C3a and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Neutralization, and Agonist and Inhibition studies.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Complement C3a/C3a (desArg) in human liver and human tonsil tissue.

Agonist or Inhibitor Analysis: Administration of C3a induces a transient influx of Ca2+ release in a dose dependent manner (fluo-3 staining on human PMNs). In this regard, it functions as an agonist for the C3a receptor (Elsner, J., et. al. (1994). Blood. 83(11):3324-31).

Neutralizing Analysis: A representative lot detected Complement C3a/C3a (desArg) in Neutralizing applications (Elsner, J., et. al. (1994). Blood. 83(11):3324-31).

Flow Cytometry Analysis: A representative lot detected Complement C3a/C3a (desArg) in Flow Cytometry applications (Elsner, J., et. al. (1994). Blood. 83(11):3324-31).

Research Category
Inflammation & Immunology

Evaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Complement C3a/C3a (desArg) in human kidney tissue sections.

187.15 kDa calculated.

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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