Skip to Content

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

For important updates on recent policy changes, please click here for more details.

Merck
CN
All Photos(6)

Key Documents

View All Documentation

MAB1637

Sigma-Aldrich

Anti-β-Tubulin III Antibody

CHEMICON®, mouse monoclonal, TU-20

Synonym(s):

Tubulin beta-III, tubulin, beta 3, tubulin, beta 4

Sign Into View Organizational & Contract Pricing

Select a Size

100 μL
CN¥6,710.12

CN¥6,710.12

Estimated to ship onApril 22, 2025Details

A recombinant, preservative-free antibody is available for your target. Try ZRB1140
Sign In for Best Price
Request a Bulk Order
All Photos(6)

Select a Size

Change View
100 μL
CN¥6,710.12

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥6,710.12

Estimated to ship onApril 22, 2025Details

A recombinant, preservative-free antibody is available for your target. Try ZRB1140
Sign In for Best Price
Request a Bulk Order

Product Name

Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1), ascites fluid, clone TU-20 (Similar to TUJ1), Chemicon®

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

TU-20 (Similar to TUJ1), monoclonal

species reactivity

rat, mouse, avian, human, bovine, monkey, pig, sheep

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
T5293CBL412XT5168
Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1) ascites fluid, clone TU-20 (Similar to TUJ1), Chemicon®

MAB1637

Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)

Monoclonal Anti-β-Tubulin antibody produced in mouse clone 2-28-33, ascites fluid

T5293

Monoclonal Anti-β-Tubulin antibody produced in mouse

Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor™488 Conjugated clone TU-20, from mouse, ALEXA FLUOR™ 488

CBL412X

Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor™488 Conjugated

Monoclonal Anti-α-Tubulin antibody produced in mouse ascites fluid, clone B-5-1-2

T5168

Monoclonal Anti-α-Tubulin antibody produced in mouse

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

TU-20 (Similar to TUJ1), monoclonal

clone

2-28-33, monoclonal

clone

TU-20, monoclonal

clone

B-5-1-2, monoclonal

species reactivity

rat, mouse, avian, human, bovine, monkey, pig, sheep

species reactivity

Ciona intestinalis, rat, chicken, Caenorhabditis elegans, Strongylocentrotus purpuratus, bovine, human

species reactivity

human, mouse, pig, bovine, primate, rat

species reactivity

mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

ascites fluid

technique(s)

ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

indirect immunofluorescence: 1:1,000 using cultured chicken fibroblasts or cultured human foreskin fibroblasts, western blot: 1:100

technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

indirect immunofluorescence: 1:2,000 using cultured human or chicken fibroblasts, radioimmunoassay: suitable, western blot: 1:4,000 using human fibroblast cell extract

General description

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins which makes up microtubules. Each has a molecular weight of approximately 55 kDa. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be evolutionarily related to tubulin.

Specificity

Expected to cross-react with Avian, Porcine (Pig), Rat, Bovine, Sheep, Human, Monkey. Reactivity with other species has not been confirmed.
The antibody reacts with the C-terminus of the neuron specific beta III isoform of tubulin. Does not identify beta tubulin found in glial cells.

Immunogen

A synthetic peptide corresponding to amino acids 443-450 (ESEAQGPK) of human class III beta-tubulin conjugated to KLH.
Epitope: C-terminus

Application

Anti-Tubulin Antibody, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) is an antibody against Tubulin for use in ELISA, IC, IH, IH(P), IP & WB with more than 55 product citations.
Immunohistochemistry:
On formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100 dilution from a previous lot. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.

Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.

Immunocytochemistry:
1:100 to 1:500 dilution from a previous lot was used on fixed cells.

Immunoblotting:
1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.

ELISA:
A previous lot of this antibody was used on ELISA.

Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Quality

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected tubulin (βIII) on 10 μg of Mouse Brain lysates.

Target description

55 kDa

Linkage

Replaces: 04-1049

Physical form

Mouse monoclonal IgG1 ascites in buffer containing PBS containing 1% BSA and 15 mM sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Positive control: Brain tissue or neurons of any type.
Negative control: Non neural tissue or Astrocytes.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

    • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

    Select a Product Number

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Function of mouse embryonic stem cell-derived supporting cells in neural progenitor cell maturation and long term expansion.
    Guan, Y; Du, QA; Zhu, W; Zou, C; Wu, D; Chen, L; Zhang, YA
    Testing null
    Yoshitaka Uno et al.
    Human gene therapy, 22(6), 711-719 (2010-12-21)
    We originally reported the use of vitamin E (α-tocopherol) as an in vivo vector of short-interfering RNA (siRNA) to the liver. Here, we apply our strategy to the brain. By combining high-density lipoprotein (HDL) as a second carrier with α-tocopherol-conjugated
    Andrew Campbell et al.
    Glia, 64(2), 227-239 (2015-10-16)
    Astroglial dysfunction plays an important role in neurodegenerative diseases otherwise attributed to neuronal loss of function. Here we focus on the role of astroglia in ataxia-telangiectasia (A-T), a disease caused by mutations in the ataxia-telangiectasia mutated (ATM) gene. A hallmark
    Shelley R Hough et al.
    PloS one, 4(11), e7708-e7708 (2009-11-06)
    Commitment in embryonic stem cells is often depicted as a binary choice between alternate cell states, pluripotency and specification to a particular germ layer or extraembryonic lineage. However, close examination of human ES cell cultures has revealed significant heterogeneity in
    Agnese Po et al.
    The EMBO journal, 29(15), 2646-2658 (2010-06-29)
    Hedgehog (Hh) pathway has a pivotal function in development and tumorigenesis, processes sustained by stem cells (SCs). The transcription factor Nanog controls stemness acting as a key determinant of both embryonic SC self-renewal and differentiated somatic cells reprogramming to pluripotency
    View All Related Papers

    Articles

    ReNcell® Human Neural Progenitors: Renewable and Consistent Human Neural Stem Cell Lines that Differentiate into Functional Neurons

    ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

    Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

    Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

    Protocols

    Neural Stem Cell Culture Protocols

    Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service