MAB13405
Anti-MMP-2 Proform Antibody, NT, clone CA-4001
clone CA-4001, Chemicon®, from mouse
Synonym(s):
Gelatinase A, 72 kDa Type IV Collagenase
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
CA-4001, monoclonal
species reactivity
rat, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MMP2(4313)
Specificity
It recognizes a protein of 72 kDa which is identified as pro (latent) form (active form is 62 kDa) of matrix metalloproteinase-2 and any fragment with N-terminal (proform) domain still attached. Antibody shows no cross-reaction with pro and active forms of MMP-9. MMP-2 is synthesized as 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids. MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). Cellular Localization: Cytoplasmic.
Immunogen
N-terminal peptide APSPIIKFPGDVAPKTDK of procollagenase IV, coupled to KLH.{Marqulies, et al 1992}
Application
Anti-MMP-2 Proform Antibody, N-terminus, clone CA-4001 detects level of MMP-2 Proform & has been published & validated for use in WB, IH(P).
Western Blot (0.5-1.0 μg/mL for 2 hours at room temperature, non-reduced conditions. However, chicken samples react best when fully reduced. Antibody dilution buffers should be simple for best results, PBS or TBS with 0.05% tween only. Immunogen used is highly conserved between species, thus immunoabsorption to pro-MMP2 in milk may impair reactivity.
Immunohistochemistry on frozen and formalin-fixed, paraffin-embedded tissue sections: 2-10 μg/mL for 30 minutes at room temperature. {Seftor, et al 2001} and Gottsch ML et al 2002}. Antigen retrieval is optional in most cases. Antibody dilution solutions should not contain serum because of possible immunoabsorption of the antibody to proMMP2 present in the solution.
Blocking: Inhibition of MMP-2 Activity (2-4 μg/mL).
Optimal working dilutions must be determined by end user.
Immunohistochemistry on frozen and formalin-fixed, paraffin-embedded tissue sections: 2-10 μg/mL for 30 minutes at room temperature. {Seftor, et al 2001} and Gottsch ML et al 2002}. Antigen retrieval is optional in most cases. Antibody dilution solutions should not contain serum because of possible immunoabsorption of the antibody to proMMP2 present in the solution.
Blocking: Inhibition of MMP-2 Activity (2-4 μg/mL).
Optimal working dilutions must be determined by end user.
Linkage
Replaces: 04-1048
Physical form
Format: Purified
Purified from ascites fluid by Protein G chromatography. Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide
Analysis Note
Control
POSITIVE CONTROL: HFL-1 cells. Conditioned, serum-free medium from (TPA-treated) human fetal lung cells. Placenta.
POSITIVE CONTROL: HFL-1 cells. Conditioned, serum-free medium from (TPA-treated) human fetal lung cells. Placenta.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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