IM29L
Anti-PAI-1 (Ab-1) Mouse mAb (2)
lyophilized, clone 2, Calbiochem®
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Synonym(s):
Anti-Plasminogen Activator Inhibitor-1
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
2, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... SERPINE1(5054)
General description
Purified mouse monoclonal antibody (see application references). Recognizes both active and latent forms of the ~50 kDa PAI-1 protein.
Recognizes both active and latent forms of the ~50 kDa PAI-1 protein in conditioned media from dexamethasone-treated HT-1080 cells. Sold under license of U.S. Patent 5,422,245.
This Anti-PAI-1 (Ab-1) Mouse mAb (2) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of PAI-1 (Ab-1).
Immunogen
Human
partially purified PAI-1 from conditioned medium of dexamethasone-treated HT1080 human cells
Application
Immunoblotting (1 µg/ml)
Immunoprecipitation (see application references)
Immunocytochemistry (see application references
Neutralization Studies (see application references)
Immunoprecipitation (see application references)
Immunocytochemistry (see application references
Neutralization Studies (see application references)
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from a volatile buffer, 100 µg BSA.
Reconstitution
We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
Analysis Note
Positive Control
Conditioned media from dexamethasone-treated (1 µM) HT-1080 cells
Conditioned media from dexamethasone-treated (1 µM) HT-1080 cells
Other Notes
Antibody should be titrated for optimal results in individual systems.
Janicke, F., et al. 1994. Cancer Research.54, 2527.
Lawrence, D. A., et al. 1994. J. Biol. Chem.269, 15223.
Nagayama, M., et al. 1994. Cancer73, 1398.
Petersen, H., et al. 1994. Cancer Res.54, 120.
Keijer, J., et al. 1991. Blood78, 401.
Blasi, F. and Verde, P., 1990. in Seminars in Cancer Biol.Vol. 1, 153.
Declerck, P. J. and Collen, D., 1990. Thrombosis Res.Supplement X 3.
Nielsen, L. S., et al. 1986. Thrombosis and Haemostasis.55, 206.
Lawrence, D. A., et al. 1994. J. Biol. Chem.269, 15223.
Nagayama, M., et al. 1994. Cancer73, 1398.
Petersen, H., et al. 1994. Cancer Res.54, 120.
Keijer, J., et al. 1991. Blood78, 401.
Blasi, F. and Verde, P., 1990. in Seminars in Cancer Biol.Vol. 1, 153.
Declerck, P. J. and Collen, D., 1990. Thrombosis Res.Supplement X 3.
Nielsen, L. S., et al. 1986. Thrombosis and Haemostasis.55, 206.
Legal Information
Sold under license of U.S. Patent 5,422,245.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Certificates of Analysis (COA)
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