HCMP1MAG-19K
MILLIPLEX® Human Complement Panel 1 - Immunology Multiplex Assay
The Human Complement Panel 1 Bead-Based Multiplex Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of complement proteins and factors in human serum, plasma and cell culture samples.
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Synonym(s):
Human complement multiplex kit, Luminex® human complement immunoassay, Millipore human complement panel
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
Recommended Products
Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 96%
(Mannose-Binding Lectin (MBL))
sensitivity: 0.0051 ng/mL
(Complement C5a)
sensitivity: 0.026 ng/mL
(Adipsin (Complement Factor D))
sensitivity: 0.040 ng/mL
(Mannose-Binding Lectin (MBL))
sensitivity: 0.22 ng/mL
(Complement Factor I)
sensitivity: 0.31 ng/mL
(Complement C2)
sensitivity: 0.36 ng/mL
(Complement C4b)
sensitivity: 1.040 ng/mL
(Complement C5)
standard curve range: 0.069-50 ng/mL
(Adipson/ Complement Factor D)
standard curve range: 0.07-50 ng/mL
(Complement C2)
standard curve range: 0.137-100 ng/mL
(Mannose-Binding Lectin (MBL))
standard curve range: 0.69-500 ng/mL
(Complement Factor I)
standard curve range: 1.37-1,000 ng/mL
(Complement C4b)
standard curve range: 2.74-2,000 ng/mL
(Complement C5)
standard curve range: 4.12-30,000 pg/mL
(Complement C5a)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
2-8°C
Related Categories
General description
The complement system consists of many plasma proteins that assist the ability of phagocytic cells and antibodies to clear pathogens. There are three different simultaneous pathways. The classical pathway is stimulated by antigen-antibody complexes; the alternative pathway spontaneously activates on contact with pathogenic cell surfaces; and the mannose-binding lectin (MBL) pathway recognizes mannose sugars usually present only on pathogenic cell surfaces. Because of the potential to be extremely damaging to host tissues, complement system activation must be tightly controlled. The regulating complement control proteins, including CD59, also known as protectin, are present at a higher concentration in the blood plasma than the complement proteins themselves. The complement system is thought to play a key role in many diseases with an immune component, such as asthma and many autoimmune diseases, including SLE, IBD and multiple sclerosis. It is also becoming increasingly associated with neurological disease, such as Alzheimer′s disease and conditions such as spinal cord injuries.
MILLIPLEX® Complement Panel 1 Bead Panel is an 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: C2, C4B, C5, C5a, Adipsin/Complement Factor D, MBL and Complement Factor I.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Immune Response
MILLIPLEX® Complement Panel 1 Bead Panel is an 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: C2, C4B, C5, C5a, Adipsin/Complement Factor D, MBL and Complement Factor I.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Immune Response
Specificity
Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Application
- Analytes: C2, C4b, C5, C5a, Adipsin/Factor D, Mannose-Binding Lectin (MBL), and Factor I
- Recommended Sample type: Serum, plasma, and tissue/cell culture supernatant
- Recommended Sample dilution: 1:200 diluted plasma or serum. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay
- Assay Run Time: Overnight or two-hour primary incubation
- Research Category: Inflammation & Immunology
- Research Subcategory: Inflammation
- Note: To avoid complement activation in samples, which will affect the results, samples should be thawed as rapidly as possible, put on ice and assayed immediately
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Packaging
96 well plate
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
WGK
WGK 3
Regulatory Information
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Certificates of Analysis (COA)
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Frank Sebastian Fröschen et al.
Diagnostics (Basel, Switzerland), 11(3) (2021-04-04)
The role and diagnostic value of the synovial complement system in patients with low-grade periprosthetic joint infection (PJI) are unclear. We sought to evaluate, for the first time, the usefulness of synovial complement factors in these patients by measuring the
Ngo Truong Giang et al.
Scientific reports, 10(1), 14923-14923 (2020-09-12)
The complement system may be crucial during dengue virus infection and progression to severe dengue. This study investigates the role of MBL2 genetic variants and levels of MBL in serum and complement proteins in Vietnamese dengue patients. MBL2 genotypes (- 550L/H
Pavan Bhargava et al.
Multiple sclerosis (Houndmills, Basingstoke, England), 27(4), 509-518 (2020-07-17)
Synaptic loss is a feature of multiple sclerosis pathology that can be seen even in normal-appearing gray matter. Opsonization of synapses with complement components may underlie pathologic synapse loss. We sought to determine whether circulating neuronal-enriched and astrocytic-enriched extracellular vesicles
Mikyle David et al.
Archives of gynecology and obstetrics, 304(6), 1467-1473 (2021-04-22)
In preeclampsia, there are excessive complement components expressed due to increased complement activation; therefore, this study investigated the concentration of adipsin and C9 in HIV-associated preeclampsia. The study population (n = 76) was stratified by pregnancy type (normotensive pregnant and preeclampsia) and
Laura McCulloch et al.
F1000Research, 8, 1039-1039 (2019-11-12)
Background: Blockade of the cytokine interleukin-1 (IL-1) with IL-1 receptor antagonist (IL-1Ra) is a candidate treatment for stroke entering phase II/III trials, which acts by inhibiting harmful inflammatory responses. Infection is a common complication after stroke that significantly worsens outcome
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