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HCYP4MAG-64K

Millipore

MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel IV

The Human Inflammation / Immunology Bead-Based Multiplex Cytokine/Chemokine Assay kit IV, using the Luminex xMAP technology, enables the simultaneous analysis of 21 cytokine and chemokine biomarkers in human serum, plasma and cell culture samples.

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Synonym(s):
Human cytokine multiplex kit, Luminex® human cytokine immunoassay, Millipore human cytokine panel
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 101%
(BAFF/Blys)
accuracy: 101%
(BRAK/CSCL14)
accuracy: 101%
(IL-28B)
accuracy: 102%
(MPIF-1/CL23)
accuracy: 106%
(IL-19)
accuracy: 110%
(HCC-4/CCL16)
accuracy: 110%
(IL-34)
accuracy: 89%
(IL-32α)
accuracy: 95%
(IL-35)
accuracy: 99%
(IL-37)
sensitivity: 0.30 ng/mL
(YKL40/CHI3L1)
sensitivity: 0.8 ng/mL
(IL-24)
sensitivity: 0.8 ng/mL
(IL-35)
sensitivity: 0.9 ng/mL
(BAFF/BLyS)
sensitivity: 10.5 ng/mL
(HMGB1/HMG1)
sensitivity: 100.8 pg/mL
(CCL28)
sensitivity: 121.5 pg/mL
(IL-34)
sensitivity: 17.2 pg/mL
(IL-28B/IFN-λ3)
sensitivity: 248.2 pg/mL
sensitivity: 27.7 pg/mL
(IL-14/α-Taxilin)
sensitivity: 28.4 pg/mL
(HCC-4)
sensitivity: 3.5 pg/mL
(IL-36β)
sensitivity: 32.1 pg/mL
(IL-32α)
sensitivity: 33.2 pg/mL
(IL-38)
sensitivity: 375.6 pg/mL
(IFNβ)
sensitivity: 4.7 pg/mL
(CXCL16)
sensitivity: 5.2 pg/mL
(IL-37/IL-1F7)
sensitivity: 6.6 pg/mL
(MIP-4/PARC)
sensitivity: 607 pg/mL
(BRAK)
sensitivity: 7.8 pg/mL
(MPIF-1)
standard curve range: 0.1-100 ng/mL
(YKL40/CHI3L1)
standard curve range: 0.44-400 ng/mL
(BAFF/Blys)
standard curve range: 0.7-750 ng/mL
(IL-24)
standard curve range: 0.8-800 ng/mL
(IL-35)
standard curve range: 10-10,000 pg/mL
(MPIF-1/CL23)
standard curve range: 2-2,000 pg/mL
(IL-36β)
standard curve range: 2.0-2,000 ng/mL
(HMGB1)
standard curve range: 24-25,000 pg/mL
(IL-14)
standard curve range: 244-250,000 pg/mL
(IL-19)
standard curve range: 29-30,000 pg/mL
(IL-28B)
standard curve range: 3-3,000 pg/mL
(CXCL16)
standard curve range: 39-40,000 pg/mL
(IL-32α)
standard curve range: 488-500,000 pg/mL
(BRAK/CXCL14)
standard curve range: 488-500,000 pg/mL
(IFNβ)
standard curve range: 49-50,000 pg/mL
(IL-38)
standard curve range: 5-5,000 pg/mL
(IL-37)
standard curve range: 5-5,000 pg/mL
(MIP-4/PARC/CCL18)
standard curve range: 98-100,000 pg/mL
(CCL28)
standard curve range: 98-100,000 pg/mL
(IL-34)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

ambient

Related Categories

General description

“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.

The MILLIPLEX® Human Cytokine / Chemokine Panel 4 enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: BAFF/Blys, BRAK/CXCL14, CCL28, CXCL16, HCC-4/CCL16, HMGB1, IFNβ, IL-14, IL-19, IL-24, IL-28B, IL-32α, IL-34, IL-35, IL-36β/IL-1F8, IL-37/IL-1F7, IL-38/IL-1F10, MIP-4, MPIF/CCL23, YKL40
  • Recommended Sample type: Serum, plasma, and tissue/cell culture supernatant
  • Recommended Sample dilution: 1:4 diluted serum or plasma. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay
  • Assay Run Time: Overnight or two-hour primary incubation assay. For best results, an overnight incubation is recommended.
  • Research Category: Inflammation & Immunology
  • NOTE: HMGB1 can be measured in serum and plasma samples only

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96 well plate

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please note: Sample Type must be selected when configuring this kit. HMGB1 can be measured in serum and plasma samples only.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

WGK

WGK 3

Regulatory Information

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Certificates of Analysis (COA)

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Carles Perpiñan et al.
Journal of personalized medicine, 12(3) (2022-03-26)
The cytokine signature present in COVID-19 could provide information on the pathogenic mechanisms of the disease and could identify possible prognostic biomarkers and possible therapeutic targets. In this longitudinal work, we studied the clinical and biochemical parameters and circulating cytokine
Henrik Hill et al.
Biomedicines, 10(1) (2022-01-22)
Gamma-aminobutyric acid (GABA) is an important inhibitory neurotransmitter in the central nervous system (CNS) and outside of the CNS, found in the highest concentrations in immune cells and pancreatic beta-cells. GABA is gaining increasing interest in diabetes research due to
Hua-Li Sun et al.
BMC infectious diseases, 21(1), 1025-1025 (2021-10-02)
The immunoregulatory functions of regulatory T cells (Tregs) in the development and progression of some chronic infectious diseases are mediated by immune checkpoint molecules and immunosuppressive cytokines. However, little is known about the immunosuppressive functions of Tregs in human brucellosis

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