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Merck
CN

GF021

Stem Cell Factor Protein, Recombinant human

The Stem Cell Factor (SCF) Protein, Recombinant human, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis.

Synonym(s):

SCF, Mast Cell Growth Factor, MGF, c-kit Ligand

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
eCl@ss:
32160405

Key Documents

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Product Name

Stem Cell Factor Protein, Recombinant human, The Stem Cell Factor (SCF) Protein, Recombinant human, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis.

biological source

human

recombinant

expressed in E. coli

assay

>98% (SDS-PAGE and HPLC)

specific activity

≥5 x 10e5 U/mg

manufacturer/tradename

Chemicon®

impurities

<0.1 ng/μg Endotoxin (of SCF; 1EU/μg)

input

sample type hematopoietic stem cell(s)

NCBI accession no.

NM_000899.3
NM_003994.4

UniProt accession no.

P21583

shipped in

dry ice

Quality Level

100

Gene Information

human ... KITLG(4254)

Related Categories

Analysis Note

Specific Activity: Chemicon′s human SCF is fully biologically active when compared to standards.The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2 ng/mL

Application

For most in vitro applications, SCF exerts its biological activity in the concentration range of 0.2 to 20.0 ng/mL. Responding cells are (partial list): Stem cells, BM precursor cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: E.coli
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant human SCF is an 18.4 kDa protein containing 165 amino acid residues corresponding to the soluble secreted form of SCF.

Physical form

The sterile filtered solution was lyophilized from 10mM Acetic acid.

Preparation Note

The lyophilized powder, though stable for a few weeks at room temperature, is best stored at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be further diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use. Reconstituted SCF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 1

Certificates of Analysis (COA)

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Qisheng Zuo et al.
EMBO reports, 23(1), e52491-e52491 (2021-11-09)
The unique characteristics of chicken primordial germ cells (PGCs) provide potential strategies for transgenic animal generation; however, insufficient PGC availability has limited their application. Regulation of bone morphogenic protein 4 (BMP4), a crucial factor for PGCs formation, may provide new
Qisheng Zuo et al.
Journal of cell science, 134(3) (2021-01-15)
The unique developmental characteristics of chicken primordial germ cells (PGCs) enable them to be used in recovery of endangered bird species, gene editing and the generation of transgenic birds, but the limited number of PGCs greatly limits their application. Studies

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