CP06
Anti-α-Tubulin Mouse mAb (DM1A)
liquid, clone DM1A, Calbiochem®
Synonym(s):
α-Tubulin Detection Antibody, Anti-Tubulin DM1A, Mouse Anti-α-Tubulin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
DM1A, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse, gerbil, chicken, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... TUBA1A(7846)
General description
Purified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa tubulin protein.
Recognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.
This Anti-α-Tubulin Mouse mAb (DM1A) is validated for use in Immunoblotting, Immunofluorescence for the detection of α-Tubulin.
Immunogen
Chicken
native chick brain microtubules
Application
Immunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references)
Immunofluorescence (5 µg/ml)
Immunofluorescence (5 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Negative Control
trp E (Ab-1)
trp E (Ab-1)
Positive Control
Any cell line
Any cell line
Other Notes
Blose, S.H., et al. 1984. J. Cell. Biol.98, 847.
Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
Tubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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