ABN101
Anti-GAD65 Antibody
from rabbit, purified by affinity chromatography
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Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human, rat, pig
species reactivity (predicted by homology)
canine (based on 100% sequence homology), porcine (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GAD2(2572)
General description
GAD65 is a group II decarboxylase enzyme that catalyzes the biosynthesis of GABA from glutamate with the aid of the cofactor, pyridoxal 5′-phosphate. GAD65 localizes at presynaptic GABAergic terminals and is reported to supply local vesicular pools of GABA facilitating transient inhibitiory neurotransmission, although GAD65 may also play a role in more sustained tonic inhibitory signals. It is present as a dimer of alpha and beta subunits and is regulated by alternative splicing, which may produce the GAD67 isoform. GAD65 also undergoes post-translational modifications such as palmitoylation of N-terminal cysteine residues and phosphorylation of multiple alpha-subunit serine residues that enable membrane-anchoring and other vesicular activities. GAD65 has been implicated in neurological conditions resulting from failure of inhibitory neurotransmission such as Huntington′s disease and epilepsy. In addition to its CNS presence, GAD65 also produces GABA in pancreatic beta cells and dysfunction in this area may contribute to the pathogenesis of insulin-dependent diabetes mellitus.
Immunogen
KLH-conjugated linear peptide corresponding to human GAD65.
Application
Anti-GAD Antibody is an antibody against GAD65 for use in Western Blotting. See below for more information about this anti GAD antibody.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Western Blot Analysis: 1 µg/mL from a representative lot detected GAD65 on 10 µg of rat brain membrane tissue lysate. Western Blot performed without Tween.
Quality
Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected GAD65 on 10 µg of rat brain membrane tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected GAD65 on 10 µg of rat brain membrane tissue lysate.
Target description
~59/63 kDa observed. The calculated molecular weight is 65 kDa, however GAD65 has been shown as a ~59/63 doublet in western blots. (Christgau, S., et al. (1991). Journal of Biological Chemistry. 266(31):21257-21264.) An uncharacterized band appears at ~38 kDa in some lysates.
Physical form
Affinity purified
Purified Rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain membrane tissue lysate
Rat brain membrane tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
recommended
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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