AB5166
Anti-Muscarinic Acetylcholine Receptor m2 Antibody
Chemicon®, from rabbit
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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CHRM2(1129)
Specificity
Recognizes a full-length m2 protein. It has exhibited no cross reactivity with other muscarinic proteins tested so far.
SPECIES REACTIVITIES: In Chimpanzee and gorilla the immunogen sequence is 100% identical. Orangutan (130/132), pig (122/132), and dog (122/132). Other species have not been tested.
SPECIES REACTIVITIES: In Chimpanzee and gorilla the immunogen sequence is 100% identical. Orangutan (130/132), pig (122/132), and dog (122/132). Other species have not been tested.
Immunogen
GST fusion protein (Rybin, 2000) and part of i3 intercellular loop of human m2 muscarinic acetylcholine receptor (amino acids 225-356) (Accession P08172).
Application
Anti-Muscarinic Acetylcholine Receptor m2 Antibody is an antibody against Muscarinic Acetylcholine Receptor m2 for use in IH, IP & WB.
Immunohistochemistry on rat brain sections.
Western blot: 1:200 using ECL on rat brain membranes.
Immunoprecipitation (Vorobiov, 2000; Lee, 2000)
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Western blot: 1:200 using ECL on rat brain membranes.
Immunoprecipitation (Vorobiov, 2000; Lee, 2000)
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Physical form
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Storage and Stability
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For positive control, in Western blot using 50 ng of protein per lane. For negative control, preincubate 5-7 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For positive control, in Western blot using 50 ng of protein per lane. For negative control, preincubate 5-7 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Anita A Disney et al.
The Journal of comparative neurology, 507(5), 1748-1762 (2008-02-12)
Acetylcholine (ACh) is believed to underlie mechanisms of arousal and attention in mammals. ACh also has a demonstrated functional effect in visual cortex that is both diverse and profound. We have reported previously that cholinergic modulation in V1 of the
Abraham Kovoor et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 25(8), 2157-2165 (2005-02-25)
Regulator of G-protein signaling 9-2 (RGS9-2), a member of the RGS family of G GTPase accelerating proteins, is expressed specifically in the striatum, which participates in antipsychotic-induced tardive dyskinesia and in levodopa-induced dyskinesia. We report that RGS9 knock-out mice develop
Tatsuya Ogura et al.
Journal of neurophysiology, 106(3), 1274-1287 (2011-06-17)
The mammalian olfactory epithelium is made up of ciliated olfactory sensory neurons (OSNs), supporting cells, basal cells, and microvillous cells. Previously, we reported that a population of nonneuronal microvillous cells expresses transient receptor potential channel M5 (TRPM5). Using transgenic mice
Zoé Christenson Wick et al.
eLife, 8 (2019-10-15)
The hippocampus, a brain region that is important for spatial navigation and episodic memory, benefits from a rich diversity of neuronal cell-types. Through the use of an intersectional genetic viral vector approach in mice, we report novel hippocampal neurons which
Naiyan Chen et al.
Nature neuroscience, 18(6), 892-902 (2015-04-29)
Cholinergic modulation of cortex powerfully influences information processing and brain states, causing robust desynchronization of local field potentials and strong decorrelation of responses between neurons. We found that intracortical cholinergic inputs to mouse visual cortex specifically and differentially drive a
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