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Key Documents

AB2200

Sigma-Aldrich

Anti-PRG4 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Megakaryocyte-stimulating factor, Superficial zone proteoglycan, articular superficial zone protein, bG174L6.2 (MSF: megakaryocyte stimulating factor ), megakaryocyte stimulating factor, proteoglycan 4, proteoglycan 4, (megakaryocyte stimulating factor,

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

human, rat, primate, horse

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NM_001127708.1

UniProt accession no.

Q92954

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PRG4(10216)

General description

PRG4, Proteoglycan 4, is a human gene and is found in synovial tissue, cartilage, and liver. Small amounts are found in the lung. Isoform 1 is found in the kidney, lung, liver, heart and brain. The other Isoforms, C & D, are widely expressed. PRG4 is a lubricating glycoprotein containing chondroitin sulfate and keratan sulfate glycosaminoglycans. It is responsible for boundary lubrication at the cartilage surface. A disease associated with PRG4 is Jacobs’s syndrome which is an autosomal recessive disorder.

Specificity

Cat. # AB2220 recognizes PRG4.

Immunogen

Epitope: C-Teminus
KLH-conjugated followed by PRG4 corresponding to the C-terminal region

Application

Anti-PRG4 Antibody is an antibody against PRG4 for use in WB & IC.
Immunocytochemistry:
Confocal IF analysis of N1E-115 using anti-PRG4 (1:500) Rabbit pAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Quality

Western Blotting

Target description

~37 kDa

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Mouse Brain lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shouan Zhu et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 37(12), 2531-2547 (2022-10-11)
Understanding how obesity-induced metabolic stress contributes to synovial joint tissue damage is difficult because of the complex role of metabolism in joint development, maintenance, and repair. Chondrocyte mitochondrial dysfunction is implicated in osteoarthritis (OA) pathology, which motivated us to study
Kosuke Oikawa et al.
Biomedical reports, 19(3), 60-60 (2023-08-24)
Suppression of the antitumor cytokine interleukin-24 (IL-24) is critical for the survival of myxoid liposarcoma (MLS) cells. It has been previously demonstrated by the authors that an MLS-specific chimeric oncoprotein, translocated in liposarcoma-CCAAT/enhancer-binding protein homologous protein (TLS-CHOP), supresses IL24 mRNA
Y Asakawa-Tanne et al.
Journal of dental research, 94(2), 337-343 (2014-12-17)
Synovial fluid of the joint decreases friction between the cartilage surfaces and reduces cartilage wear during articulation. Characteristic changes of synovial fluid have been shown in patients with osteoarthritis (OA) in the temporomandibular joint (TMJ). OA is generally considered to

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