biological source
goat
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... VIM(7431)
Specificity
Recognizes vimentin in cultured mammalian cells and tissue sections. AB1620 specifically stains the vimentin band (58 kD) using total cell or tissue extracts in immunoblotting. No reactivity to desmin, keratin, neurofilaments or glial filaments. Shows wide cross-reactivity among mammalian species using various immunolabeling techniques.
Immunogen
Vimentin purified from cultured human foreskin fibroblasts.
Application
Anti-Vimentin Antibody detects level of Vimentin & has been published & validated for use in IC, IH, IH(P) & WB.
Immunohistochemistry: 1:20-1:40. Shows reactivity on frozen tissue sections as well as formalin-fixed paraffin embedded tissue by immunoperoxidase and immunofluorescent assays.
Immunocytochemistry
Western blot
Optimal working dilutions must be determined by end user.
Immunocytochemistry
Western blot
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Target description
58 kDa
Physical form
Neat goat Serum. Liquid containing 0.1% sodium azide.
Unpurified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Breast carcinoma tissue, Colon, Prostate, Tonsil, Tonsilar lymphoma, Uterus, Ureter
Breast carcinoma tissue, Colon, Prostate, Tonsil, Tonsilar lymphoma, Uterus, Ureter
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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L F Horowitz et al.
NPJ precision oncology, 4, 12-12 (2020-05-22)
Current methods to assess the drug response of individual human cancers are often inaccurate, costly, or slow. Functional approaches that rapidly and directly assess the response of patient cancer tissue to drugs or small molecules offer a promising way to
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Hyperhomocysteinemia (Hhcy), or increased levels of the excitatory amino acid homocysteine (Hcy), is implicated in glaucoma, a disease characterized by increased oxidative stress and loss of retinal ganglion cells (RGCs). Whether Hhcy is causative or merely a biomarker for RGC
Karen A Sap et al.
Molecular & cellular proteomics : MCP, 18(9), 1705-1720 (2019-05-30)
Huntington's disease is caused by a polyglutamine repeat expansion in the huntingtin protein which affects the function and folding of the protein, and results in intracellular protein aggregates. Here, we examined whether this mutation leads to altered ubiquitination of huntingtin
DNA-PK target identification reveals novel links between DNA repair signaling and cytoskeletal regulation.
Kotula, E; Faigle, W; Berthault, N; Dingli, F; Loew, D; Sun, JS; Dutreix, M; Quanz, M
Testing null
Jing Wang et al.
Free radical biology & medicine, 86, 25-36 (2015-04-30)
Oxidative stress figures prominently in retinal diseases, including diabetic retinopathy, and glaucoma. Ligands for σ1R, a unique transmembrane protein localized to the endoplasmic reticulum, mitochondria, and nuclear and plasma membranes, have profound retinal neuroprotective properties in vitro and in vivo.
Protocols
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
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