biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
human, marmoset
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
unmodified
Gene Information
human ... MMP9(4318)
General description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes all forms of MMP-9 under reducing and non-reducing conditions.
Recognizes all forms of MMP-9 under reducing and non-reducing conditions. Exhibits only slight cross-reactivity with MMP-2. Does not cross-react with MMP-1, MMP-3, or MMP-8.
This Anti-MMP-9 Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of MMP-9.
Immunogen
human pro-MMP-9
Application
ELISA (see comments)
Immunoblotting (1:500-1:1000)
Immunofluorescence (1:50-1:250)
Immunohistochemistry (1:40-1:150)
Immunoprecipitation (1:20-1:60)
Immunoblotting (1:500-1:1000)
Immunofluorescence (1:50-1:250)
Immunohistochemistry (1:40-1:150)
Immunoprecipitation (1:20-1:60)
Warning
Toxicity: Standard Handling (A)
Physical form
Undiluted serum.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Exhibits very slight reactivity with MMP-2. Does not cross-react with collagenases, MMP-1, MMP-3, or MMP-8. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
Kolkenbrock, H., et al. 1996. Biol. Chem.377, 529.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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