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Supelco

Chromolith® HighResolution RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 5 mm x 4.6 mm Guard Cartridges (3 pieces)

Synonym(s):

Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces)

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

Chromolith® HighResolution RP-18 endcapped, L × I.D. 5 mm x 4.6 mm guard cartridges (3 pieces)

material

PEEK column

Quality Level

Agency

suitable for ISO/CEN 6304.09
suitable for USP L1

product line

Chromolith®

feature

endcapped
endcapped

packaging

pkg of 3 ea

extent of labeling

15% Carbon loading
18% Carbon loading

parameter

200 bar pressure
400 bar pressure
45 °C max. temp.
50 °C max. temp.

technique(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)

column L × I.D.

0.5 cm × 4.6 mm

surface area

250 m2/g × 250 m2/g

matrix

highly porous
monolithic silica
monolithic silica

matrix active group

C18 bonding phase, octadecylsilane (ODS)
C18 bonding phase

pore size

1.15 μm macroporosity
1.5 μm macroporosity
15 nm mesoporosity
15 nm mesoporosity

operating pH range

2-7.5
2-7.5

separation technique

reversed phase

storage temp.

no temp limit

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General description

Although the Chromolith® columns are well known for their robustness and longevity, guard columns are available to prolong the lifespan of Chromolith® analytical columns even further. These guard cartridges require a special holder.

Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges are extremely easy to use. The guard cartridges are simply added directly in front of the main column to protect it from chemical or mechanical contamination. Due to the benefits of monolithic technology, and the convenience of Chromolith® guard columns, they are also popular for use with classical particulate columns. Moreover, guard columns can be used as trap columns when large sample volumes are to be injected. Guard columns should be changed frequently in order to avoid excessive accumulation of impurities.

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges are used with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Application


  • A Novel and Sensitive LC-MS/MS Method for the Quantitation of Ceftiofur in Pharmaceutical Preparations and Milk Samples.: This study, conducted by Levent S, Özcan S, Geven A, and Can NÖ, presents a new LC-MS/MS method utilizing Chromolith® HighResolution RP-18 endcapped columns. The method provides high sensitivity and specificity for the quantitation of ceftiofur, a veterinary antibiotic, in both pharmaceutical preparations and milk samples, demonstrating its potential for robust analytical applications in pharmaceutical and food safety contexts (Levent et al., 2021).

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

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