1.52003
Chromolith® RP-18 endcapped (monolithic) HPLC Columns
L × I.D. 25 mm x 3 mm HPLC Column
Synonym(s):
Chromolith® Flash RP-18 endcapped 25-3 HPLC column
About This Item
product name
Chromolith® RP-18 endcapped, L × I.D. 25 mm x 3 mm HPLC column
material
PEEK column
Quality Level
Agency
suitable for USP L1
product line
Chromolith®
feature
endcapped
packaging
1 ea of
extent of labeling
18% Carbon loading
parameter
200 bar pressure
45 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
2.5 cm × 3 mm
matrix
monolithic silica
matrix active group
C18 bonding phase
pore size
13 nm mesoporosity
2 μm macroporosity
operating pH range
2-7.5
separation technique
reversed phase
storage temp.
no temp limit
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General description
Chromolith® Flash RP-18 endcapped 25-3 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® Flash RP-18 endcapped 25-3 columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® Flash RP-18 endcapped 25-3 columns are perfectly suitable for LC-MS applications.
The column dimension of 25 mm length and 3 mm I.D. is the best choice for rapid separations on any HPLC and UHPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-3 guard cartridges (3 pieces) [1.52005] with the Chromolith® Guard Cartridge Holder 5 mm x 3 mm [1.52004]
Holder and guard cartridges are also available as Guard cartridge set [1.52008]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Analysis Note
Pressure: ≤ 10 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3.3 - 4.3
Tailing (USP): 0.90 - 1.55 %
Legal Information
Certificates of Analysis (COA)
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Articles
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
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