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Supelco

Purospher® STAR RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 4 mm x 4 mm LiChroCART® Guard-cartridge

Synonym(s):

Purospher® STAR RP-18 endcapped (5 µm) LiChroCART® 4-4

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

Purospher® STAR RP-18 endcapped (5 µm), L × I.D. 4 mm x 4 mm LiChroCART® Guard-cartridge

material

stainless steel column

Quality Level

100

Agency

suitable for USP L1

product line

Purospher®

feature

endcapped

packaging

10 pak of

extent of labeling

17% Carbon loading

parameter

250 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

0.4 cm × 4 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C18 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase

storage temp.

no temp limit

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General description

Purospher® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR RP-18 endcapped, 5 µm LiChroCART® 4-4 is a guard cartridge which allows the integration into a manuCART™ cartridge column holder part number 1.51486.0001 for direct, dead volume free connection to a analytical LiChroCART® cartridge column with inner dimension or 2, 3, 4, or 4.6 mm I.D. and a column length of 75, 100, 125, 150 or 250 mm.For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART® cartridges, part number 1.51487.0001 can be used.For connection to any other analytical HPLC column with capillary connection the Precolumn holder for 4-4 LiChroCART® cartridges, part number 1.16217.0001 is suitable.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Analysis Note

Selectivity: passes test

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tanaka Test for C18 Stationary Phase in HPLC Columns

Discover the Tanaka Test, a key protocol for evaluating the performance and selectivity of stationary phases in Reversed Phase HPLC separations.

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