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14-902-M

Sigma-Aldrich

AMPK (α2, β1, γ1) Protein, active, 10 µg

Active, recombinant, human AMPK (α2, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα2, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

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UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

100

recombinant

expressed in E. coli

product line

Upstate®

specific activity

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)

mol wt

Mw 30 kDa (AMPKß1)
Mw 38 kDa (AMPKγ1)
Mw 63 kDa (AMPKα2)

purified by

(Ni2+/NTA-agarose)

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

accession no.

 (Function: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5′-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. )
NM_006252

NCBI accession no.

NM_002733
NM_006252
NM_006253

UniProt accession no.

P54619
P54646
P54646, Q9Y478, P54619
Q9Y478

relevant disease(s)

diabetes; obesity; metabolic disorders

Gene Information

human ... PRKAA2(5563) , PRKAB1(5563)

General description

Recombinant, human AMPK (α2, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα2, full length untagged AMPKβ1 and full length untagged AMPKγ1.

Application

Research Category
Metabolism

Apoptosis & Cancer
Research Sub Category
Diabetes

Obesity

Metabolic Disorders

Biochem/physiol Actions

Protein Target: AMPK (α2, β1, γ1)
Target Sub-Family: CAMK

Packaging

Also available in 10 μg size and in bulk
Also available in 250µg size as catalog # 14-902M - Call for pricing and availability.

Quality

Routinely evaluated by phosphorylation of substrate peptide

Target description

AMPK (α2, β1, γ1)

Physical form

Cobalt-agarose

Storage and Stability

6 months at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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