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14-638-M

Sigma-Aldrich

MLCK Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human MLCK, amino acids 1425-1771, for use in Kinase Assays.

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UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 43.7 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

UniProt accession no.

Gene Information

human ... MYLK(4638)

General description

N-terminal 6His-tagged, recombinant, human MLCK, amino acids 1425-1771
Product Source: expressed by baculovirus in Sf21 cells

Application

Research Category
Inflammation & Immunology

Biochem/physiol Actions

Protein Target: MLCK
Target Sub-Family: CAMK

Quality

routinely evaluated by phosphorylation of ZIPtide (Catalog # 12-545)

Physical form

Ni2+/NTA-agarose

Storage and Stability

6 months at -70°C from date of shipment

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Myosin light chain kinase stimulates smooth muscle myosin ATPase activity by binding to the myosin heads without phosphorylating the myosin light chain.
Gao, Ying, et al.
Biochemical and biophysical research communications, 305, 16-21 (2003)

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