14-476-M
Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg
Active complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged & recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.
Synonym(s):
CDKN7, Cell division protein kinase 7, MO15, STK1, Serine/threonine-protein kinase 1
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41
biological source
human
Quality Level
recombinant
expressed in baculovirus infected Sf21 cells
form
liquid
shelf life
1 yr
mol wt
Mw 37.8 kDa (cyclin H)
Mw 39.9 kDa (cdk7)
Mw 62.8 kDa (MAT1)
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
solubility
soluble
NCBI accession no.
UniProt accession no.
storage temp.
−70°C
Gene Information
human ... CDK7(1022)
General description
Research area: APOPTOSIS
Protein Target: CDK7/cyclinH/MAT1 Target Sub-Family: CMGC
Complex of recombinant full length human cdk7, containing a C-terminal His6-tag, recombinant full-length human cyclin H untagged and recombinant full-length human MAT1 containing an N-terminal GST-tag Product Source: All are expressed by baculovirus in Sf21 insect cells
Cdk-activating kinase (CAK) is a trimeric complex composed of cyclin-dependent kinase 7 (Cdk7), cyclin H, and RING finger protein Methionine Adenosyltransferase 1 (MAT1). Cyclin H activates Cdk7, while MAT1 plays a role in influencing the substrate specificity of the complex.
Protein Target: CDK7/cyclinH/MAT1 Target Sub-Family: CMGC
Complex of recombinant full length human cdk7, containing a C-terminal His6-tag, recombinant full-length human cyclin H untagged and recombinant full-length human MAT1 containing an N-terminal GST-tag Product Source: All are expressed by baculovirus in Sf21 insect cells
Cdk-activating kinase (CAK) is a trimeric complex composed of cyclin-dependent kinase 7 (Cdk7), cyclin H, and RING finger protein Methionine Adenosyltransferase 1 (MAT1). Cyclin H activates Cdk7, while MAT1 plays a role in influencing the substrate specificity of the complex.
Biochem/physiol Actions
The Cdk7/cyclin H/Mat1 (CAK complex) plays a role in both cell cycle regulation and transcription. Within the complex, Cdk7 functions as the Cdk-activating kinase for cell cycle and as a part of the general transcription factor, Transcription Factor II H (TFIIH), for transcription process. Additionally, it also regulates gene expression by phosphorylating transcription factors such as estrogen receptor-α (ER), p53, retinoid receptors, androgen receptor, and estrogen receptor. Elevated expression levels of Cdk7, cyclin H, and MAT1 have been observed in breast cancer patients. Inhibition of cdk7 is associated with the development of colorectal cancer in humans.
Packaging
Also available in 250μg size, please inquire for pricing and availability.
Quality
routinely evaluated by phosphorylation of cdk7 substrate peptide
Physical form
Ni2+/NTA-agarose
Storage and Stability
1 year at -70°C
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Expression of CDK7, Cyclin H, and MAT1 Is Elevated in Breast Cancer and Is Prognostic in Estrogen Receptor-Positive Breast Cancer
Patel H, et al.
Clinical Cancer Research, 22(23), 5929-5938 (2016)
Functional analysis of the Cdk7.cyclin H.Mat1 complex in mouse embryonic stem cells and embryos
Shetal A Patel and M Celeste Simon
Journal of Separation Science, 285(20), 15587-15598 (2010)
Eric J Tomko et al.
Journal of molecular biology, 433(14), 166813-166813 (2021-01-17)
The general transcription factor TFIIH contains three ATP-dependent catalytic activities. TFIIH functions in nucleotide excision repair primarily as a DNA helicase and in Pol II transcription initiation as a dsDNA translocase and protein kinase. During initiation, the XPB/Ssl2 subunit of
DK regulators?Cell cycle progression or apoptosis?Scenarios in normal cells and cancerous cells
D'costa M, et al.
Advances in Protein Chemistry and Structural Biology, 135 (2023)
Tinggui Yin et al.
Molecular cancer therapeutics, 13(6), 1442-1456 (2014-04-02)
DNA-dependent RNA polymerase II (RNAP II) largest subunit RPB1 C-terminal domain (CTD) kinases, including CDK9, are serine/threonine kinases known to regulate transcriptional initiation and elongation by phosphorylating Ser 2, 5, and 7 residues on CTD. Given the reported dysregulation of
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