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Cellvento® 4HEK Liquid

Chemically defined, sterile; sterile-filtered, suitable (for HEK293 cell culture)

Pharma Manufacturing

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Quality Level

500

description

shipped with cooling pack

sterility

sterile; sterile-filtered

product line

Cellvento®

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

storage temp.

2-8°C

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General description

Cellvento® 4HEK Medium is a chemically defined medium optimized for adeno-associated virus (AAV) production in multiple human embryonic kidney (HEK) 293 lineages. Cellvento® 4HEK Medium has been streamlined and cell culture media components incorporated to increase media stability and reduce protein oxidation. The formulation is animal component free.

Application

Cellvento® 4HEK Medium can be used as amplification and production medium for gene therapy applications. This medium enables low doubling time and high viable cell densities with multiple HEK293 cell lines. Cellvento®4HEK Medium is compatible with commonly used transfection reagents like PEI, supports AAV production and facilitates high titers of full capsids in various HEK293 lineages. This easy-to-use medium can be used for all stages like growth, transfection, and production.

Quantity

Formulated to contain 30.8 grams of powder per liter of medium.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

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Articles

Development of a Novel Cell Culture Medium for AAV Production With Multiple HEK293 Lineages and Process Optimization

Read our white paper on the development of a novel cell culture medium for AAV production with multiple HEK293 lineages and process optimization.

Scalability Challenges of Viral Vector Manufacturing

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Can AAV Continue to Deliver the Promise of Gene Therapy?

The current status and future manufacturing of AAV-based gene therapies is discussed in this podcast transcript, including how to streamline large-scale manufacturing.

Cell and Gene Therapy Manufacturing: Upstream Processing for AAV and Lentivirus Platforms

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A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale.

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