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1.08487

Supelco

Urea

GR for analysis ACS,Reag. Ph Eur

Synonym(s):

Carbamide, Carbonyl diamide, Diaminomethanone, Carbonyldiamine, Carbamide, Carbonyldiamide

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About This Item

Linear Formula:
NH2CONH2
CAS Number:
57-13-6
Molecular Weight:
60.06
Beilstein:
635724
MDL number:
MFCD00008022
UNSPSC Code:
12352111
EC Index Number:
200-315-5
NACRES:
NA.21

grade

ACS reagent

Quality Level

200

Agency

reag. Ph. Eur.

vapor pressure

<0.1 hPa ( 20 °C)

form

solid

potency

8471 mg/kg LD50, oral (Rat)
8200 mg/kg LD50, skin (Rat)

technique(s)

titration: suitable

pH

9 (20 °C, 100 g/L in H2O)

mp

132-135 °C (lit.)

density

1.335 g/mL at 25 °C (lit.)

bulk density

720‑760 kg/m3

storage temp.

15-25°C

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

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Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Linkage

Replaces: UX0065-1

Analysis Note

Assay (ex N): 99.0 - 100.5 %
Assay (ex N, calc. on dried substance): 99.0 - 101.5 %
Purity (DSC (differential scanning calorimetry)): ≥ 99.5 %
Identity (IR-spectrum): passes test
Appearance of solution (10 %; Wasser; colour): colourless
Appearance of solution (10 %; Wasser; clarity): clear
In water insoluble matter: ≤ 0.003 %
Acidity, Alkalinity: ≤ 0.0005 meq/g
Melting point (DSC): 132 - 135 °C
NH₄ (Ammonium): ≤ 0.0500 %
Chloride (Cl): ≤ 0.0005 %
Sulfate (SO₄): ≤ 0.001 %
Heavy metals (as Pb): ≤ 0.0004 %
Biuret: ≤ 0.05 %
Sulfated ash (600 °C): ≤ 0.005 %
Cu (Copper): ≤ 0.0001 %
Fe (Iron): ≤ 0.0001 %
Pb (Lead): ≤ 0.0002 %
Loss on Drying (105°C): ≤ 1.0 %
Corresponds to ACS, Reag. Ph Eur

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

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Protocols

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