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Millipore

ChromoCult® Listeria Agar Enrichment-Supplement

ChromoCult®, selective for Listeria spp.

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Synonym(s):
Listeria Enrichment Supplement
UNSPSC Code:
41106212
NACRES:
NA.74

Agency

APHA
ISO 11133
ISO 11290-1
ISO 11290-2

Quality Level

100

form

lyophilized powder

manufacturer/tradename

ChromoCult®

technique(s)

microbiological culture: suitable

pH

6.6 (20 °C in H2O)

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

2-8°C

suitability

selective for Listeria spp. (Agar)

Related Categories

General description

ChromoCult® Listeria Agar Enrichment-Supplement is a homogeneous, sterile suspension of L-α-phosphatidylinositol in distilled water. Differentiation of Listeria monocytogenes from non-pathogenic Listeria sp. is achieved through the production of a phosphatidylinositol-specific phospholipase C (PI-PLC). Listeria monocytogenes hydrolyses the specific purified substrate added to the medium producing an opaque halo around the colonies.

Application

ChromoCult® Listeria Agar Enrichment-Supplement is used as an additive for the preparation of ChromoCult® Listeria Selective Agar for detection and enumeration of Listeria monocytogenes and of Listeria sp. in a variety of food products.

Linkage

Replaces: 1004390017

Analysis Note

Growth promotion test according to ISO 11133.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
≥ 70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 Std. 37°C aerobic; other strains at least 48 hrs.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Certificates of Analysis (COA)

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Articles

Detection of Listeria monocytogenes in Food and Environmental Samples

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

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