07-789
Anti-phospho-AKT (Thr34) Antibody
Upstate®, from rabbit
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Synonym(s):
Akt1, PKB, PRKBA, RAC, RAC-ALPHA
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pThr34)
Gene Information
human ... AKT1(207)
Specificity
Predicted to cross react with Mouse and Rat due to high sequence homology, but have not been tested.
Recognizes phospho-AKT (Thr34)
Immunogen
KLH conjugated peptide corresponding to amino acid region encompassing the human phospho-AKT (Thr34); Sequence highly conserved in mouse and rat.
Application
Anti-phospho-AKT (Thr34) Antibody detects level of phospho-AKT (Thr34) & has been published & validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
General Post-translation Modification
PI3K, Akt, & mTOR Signaling
General Post-translation Modification
Quality
Routinely evaluated by immunoblot.
Target description
60 kDa
Physical form
100 μL of epitope specific affinity purified rabbit polyclonal IgG in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
ImmunoAffinity Purified
Storage and Stability
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond.
Marte, B M and Downward, J
Trends in Biochemical Sciences, 22, 355-358 (1997)
Cisplatin activates Akt in small cell lung cancer cells and attenuates apoptosis by survivin upregulation
Belyanskaya, Larisa L, et al
International Journal of Cancer. Journal International Du Cancer, 117, 755-763 (2005)
Virginia Actis Dato et al.
Cells, 9(1) (2020-01-16)
Abstract: The cardiovascular disease (CVD) frequently developed during metabolic syndrome and type-2 diabetes mellitus is associated with increased levels of aggregation-prone small LDL particles. Aggregated LDL (aggLDL) internalization is mediated by low-density lipoprotein receptor-related protein-1 (LRP1) promoting intracellular cholesteryl ester
Virginia Actis Dato et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 152, 113270-113270 (2022-06-17)
Antibodies against the P3 sequence (Gly1127-Cys1140) of LRP1 (anti-P3 Abs) specifically block cholesteryl ester (CE) accumulation in vascular cells. LRP1 is a key regulator of insulin receptor (InsR) trafficking in different cell types. The link between CE accumulation and the
Progesterone receptor enhances breast cancer cell motility and invasion via extranuclear activation of focal adhesion kinase.
Fu, XD; Goglia, L; Sanchez, AM; Flamini, M; Giretti, MS; Tosi, V; Genazzani, AR; Simoncini, T
Endocrine-Related Cancer null
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