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Sigma-Aldrich

Anti-eNOS/NOS III Antibody, CT

Upstate®, from rabbit

Synonym(s):

Constitutive NOS, Endothelial NOS, Endothelial NOS, Endothelial NOS, endothelial nitric oxide synthase, nitric oxide synthase 3 (endothelial cell)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, pig, guinea pig, rat, canine, human, bovine

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NP_000594

UniProt accession no.

P29474

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

bovine ... Nos3(287024)
dog ... Nos3(403784)
guinea pig ... Nos3(100135577)
human ... NOS3(4846)
mouse ... Nos3(18127)
pig ... Nos3(397557)
rat ... Nos3(24600)

General description

Endothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells.

Specificity

Predicted to cross-react with mouse, guinea pig, pig, rat and dog based on sequence homology.
Recognizes eNOS/NOS III, Mr ~135 kDa.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, pig, rat, and dog.

Application

Anti-eNOS/NOS III Antibody, CT detects level of eNOS/NOS III & has been published & validated for use in WB.

Quality

Routinely evaluated by western blot in RIPA lysates from HUVEC cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells.

Target description

135 kDa

Physical form

Format: Purified
Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.

Analysis Note

Control
Platelets, placenta, liver and kidney.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

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Daniela Palmieri,Patrizia Perego,Domenico Palombo
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Caroline Brunetto de Farias,Denis Broock Rosemberg,Tiago Elias Heinen et al.
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Fetal pulmonary vascular remodeling in a rat model induced by hypoxia and indomethacin.
Xue-Feng Xu,Wei-Zhong Gu,Xi-Ling Wu,Ru-Yi Li,Li-Zhong Du
The Journal of Maternal-Fetal & Neonatal Medicine : The Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians null
The senescence-accelerated mouse (SAM-P8) as a model for the study of vascular functional alterations during aging.
Llorens, Silvia, et al.
Biogerontology, 8, 663-672 (2007)
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