Anti-α-Tubulin Antibody, clone DM1A

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Recognizes α-Tubulin, clone DM1A, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to amino acids 426-450.

Epitope: a.a. 426-450

Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSVEGEGEEEGEE).

Anti-α-Tubulin Antibody, clone DM1A is an antibody against α-Tubulin for use in IC, IF & WB.

Immunofluorescence:
(Breitling, F., 1986)

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Routinely evaluated by western blot on non-stimulated A431 lysate.

Western Blot Analysis:
0.1-1 µg/mL of this lot detected α- Tubulin in RIPA lysates from A431, L6 cells and rat whole brain tissue.

50-60 kDa

Replaces: 04-1117

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide and 30% glycerol.

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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