03-0176-00
SMC® Human IL-10 High Sensitivity Immunoassay Kit
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species reactivity
rat, mouse, feline, canine, nonhuman primates
manufacturer/tradename
SMC®
assay range
sensitivity: 0.10 pg/mL
(Lower Limit of Quantification:)
inter-assay cv: <20%
intra-assay cv: <15%
(Intra-assay n=20; Inter-assay n=5)
technique(s)
single molecule counting: suitable
storage temp.
2-8°C
Related Categories
General description
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
Specificity
Although IL-10 was detectable in non-human primate, mouse, rat, canine, and feline plasma, the assay has not been validated for these species.
Accuracy:
- Minimum of 5 serum samples spiked with average recovery of 87% with a range of 84-93%
- Minimum of 5 plasma samples spiked with average recovery of 90% with a range of 82-97%
Application
This new streamline kit has been improved with the following features including new including new improvements over the legacy IL-10 kit, #03-0056-00:
- Improved LLOQ--0.10 pg/mL in the new streamlined kit while the legacy kit is 0.39 pg/mL
- Adjusted standard curve--changed to 0.1 – 100 pg/mL from 0.17 – 400 pg/mL
- Improved options for sample prep and incubation
- Lyophilization of the standard provides the kit at one storage condition
- Removal of a plate transfer step
- Increased reagent volumes to accommodate automation
- Validated to run on both SMCxPRO® and Erenna
Research Category:
Inflammation & Immunology
Research Sub-Category:
- Inflammatory Disease
- Immune Response
- Autoimmune disease
Packaging
Components
- Assay Buffer
- IL-10 Coated Beads
- Standard Diluent
- IL-10 Detection Antibody
- IL-10 Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
WGK
WGK 3
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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